Tumor Necrosis Factor-α-Induced Colitis Increases NADPH Oxidase 1 Expression, Oxidative Stress, and Neutrophil Recruitment in the Colon: Preventive Effect of Apocynin
Figure 6
TNFα-induced colitis results in an increase of oxidative stress markers in the colon, an effect that is prevented by apocynin. (a) Superoxide anion release was evaluated by NBT reduction in fresh colon tissues of mice treated with TNFα (10 μg · kg−1, ip) in the presence or absence of apocynin (25 mg · kg−1, ip) as described in Section 2. Data are expressed as percentage of control absorbance at 560 nm (control mice treated with saline). Means ± S.E.M from mice in each group. versus control group; and ; versus TNFα-treated group; (b) lipid peroxidation was assessed by malondialdehyde (MDA) levels in fresh colon tissues of mice treated with TNFα (10 μg · kg−1, ip) in the presence or absence of apocynin (25 mg · kg−1, ip) as described in Section 2. Data are expressed as means ± S.E.M from mice in each group. , , and versus control; , , and versus TNFα.
