Tumor Necrosis Factor-α-Induced Colitis Increases NADPH Oxidase 1 Expression, Oxidative Stress, and Neutrophil Recruitment in the Colon: Preventive Effect of Apocynin
Figure 8
Redox-sensitive MAP kinases are activated in the colon of TNFα-treated mice: modulation by apocynin. (a) Upper: ERK1/2 activation was assessed by Western blot using phospho-ERK1/2 specific antibodies in colon of mice treated with TNFα (10 μg · kg−1, ip) in the presence or absence of apocynin (25 mg · kg−1, ip) (C in the figure is for control untreated mice). Total ERK1/2 protein, assessed with an ERK1/2 antibody, was used as loading control. Lower: densitometric analysis of the ratio of phospho-ERK1/2 to total ERK1/2 protein. Data are expressed as means ± S.E.M from mice in each group. , , and versus control; , , and versus TNFα. (b) Upper: p38MAPK activation was assessed by Western blot using phospho-p38MAPK specific antibodies in colon of mice treated with TNFα (10 μg · kg−1, ip) in the presence or absence of apocynin (25 mg · kg−1, ip) (C in the figure is for control untreated mice). Total p38MAPK protein, assessed with a p38MAPK antibody, was used as loading control. Lower: densitometric analysis of the ratio of phospho-p38MAPK to total p38MAPK protein. Data are expressed as means ± S.E.M from mice in each group. , , and versus control; , , and versus TNFα. (c) Upper: JNK activation was assessed by Western blot using phospho-JNK specific antibodies in colon of mice treated with TNFα (10 μg · kg−1, ip) in the presence or absence of apocynin (25 mg · kg−1, ip) (C in the figure is for control untreated mice). Total JNK protein, assessed with a JNK antibody, was used as loading control. Lower: densitometry analysis of the ratio of phospho-JNK to total JNK. Data are expressed as means ± S.E.M from mice in each group. , , and versus control; , , and versus TNFα.
