MicroRNA-146a Decreases High Glucose/Thrombin-Induced Endothelial Inflammation by Inhibiting NAPDH Oxidase 4 Expression
Figure 5
The stimulatory effects of HG/thrombin on Nox4 expression and ROS production were attenuated in miR-146a mimic-transfected HAECs. (a) The stimulatory effect of HG/thrombin on Nox4 mRNA expression was preserved in miR-control-transfected HAECs, with an increase of 8.95-fold Nox4 mRNA levels compared with the control. In contrast, HG/thrombin did not increase Nox4 mRNA expression in miR-146a mimic-transfected HAECs. The bars represent the means ± SEM from 3 experiments. , as compared with the miR-control. (b) The stimulatory effect of HG/thrombin on Nox4 protein expression was significantly attenuated in miR-146a mimic-transfected HAECs. The bars represent the means ± SEM from 4 experiments. , as compared with the miR-control. (c) The stimulatory effect of HG/thrombin on intracellular ROS production was significantly attenuated in miR-146a mimic-transfected, HG/thrombin-stimulated HAECs. The bars represent the means ± SEM from 3 experiments. , as compared with the miR-control.
