Low Intraprostatic DHT Promotes the Infiltration of CD8+ T Cells in BPH Tissues via Modulation of CCL5 Secretion
Induction of chemokines in BPH-1 cells stimulated by changes of DHT level. (a) Q-PCR screening of a panel of cytokine factors that could be responsible for BPH-1 cell promoted T-cell migration. Compared to the BPH-1 cells cultured with normal medium, mRNA transcription of CCL5 was upregulated in BPH-1 cells with charcoal medium treatment; (-test). (b) Transcription of chemokine related receptors mRNA was detected by q-PCR. (ANOVA and Newman-Keuls test). (c) The interruption assay by adding CCL5 neutralizing antibody in above migration system. Data presented as the average cell numbers in lower chamber (mean ± SEM). (ANOVA and Newman-Keuls test).