|
| Studies | Disease | Sample | Method |
Results |
Localization |
|
| Ajduković et al., [22] (2010) | UC | Serum | ELISA | IL-33 not increased compared to controls | NA |
|
| Kobori et al., [15] (2010) | UC
CD | Colonic biopsies | qPCR
IHC | ↑IL-33 in active UC | Subepithelial myofibroblasts |
|
|
Pastorelli et al., [16] (2010) |
UC
CD | Colonic biopsies | qPCR
WB | ↑IL-33 in active IBD
(UC > CD) | NA |
| Surgical specimens | IHC | ↑IL-33 in active UC | Intense staining mainly localized to the epithelium and infiltrating LPMC |
| IEC isolated from surgical specimens | qPCR
WB | ↑IL-33 in active UC | IL-33 is predominantly expressed by IEC in active UC |
| Serum | ELISA
WB | ↑IL-33 in active IBD
Only cleaved IL-33 in sera | NA |
|
| Seidelin el al., [17] (2010) | UC | IEC isolated from biopsies | qPCR
WB | ↑IL-33 in active > inactive > controls | Localized in the epithelium and infiltrating lymphocytes |
|
|
Beltrán et al., [18] (2010) |
UC
CD | Serum | ELISA | ↑IL-33 in IBD patients | NA |
| Colonic biopsies | ELISA
IF | ↑IL-33 in active IBD | In controls and CD, IL-33 was localized in the cytoplasm of epithelial cells. In UC, a decreased cytoplasm staining was observed. Both IBD showed strong nuclear staining |
|
|
Sponheim et al., [19] (2010) |
UC
CD | Colonic biopsies | qPCR | ↑IL-33 in UC | NA |
| Surgical specimens | IHC | Nuclear expression was seen only rarely in crypts of IBD samples | In UC, focal accumulation of cells with IL-33-positive nuclei underlying ulcerations was found (myofibroblasts) |
|
| Sedhom et al., [20] (2012) | UC
CD | Surgical specimens | qRT-PCR
ELISA
IHC
WB | ↑IL-33 in active colonic tissue versus noninvolved areas | In involved mucosa, nuclear IL-33 was found in colonic epithelial cells. In CD, inflammatory aggregates were found surrounding IL-33+ cells. In UC, IL-33+ cells formed “shield-like” clusters in ulcers |
|
| Wakahara et al., [21] (2012) | UC
CD | Colonic explant culture | ELISA | ↑IL-33 in IBD patients.
No difference between active versus inactive sites | NA |
|
