Vitamin C Mitigates Oxidative Stress and Tumor Necrosis Factor-Alpha in Severe Community-Acquired Pneumonia and LPS-Induced Macrophages
Figure 3
The effect of vitamin C on ROS, DNA damage, TNF-α, and IL-6 in vitro. Monocytes from severe CAP were treated with vitamin C for 12 h in vitro. (a) Intracellular ROS were detected by confocal microscope using DHE probe (×400). DNA damage was detected by comet assay using confocal microscope (×400). (b) Whole blood cells from severe CAP were treated with vitamin C for 12 h in vitro. Standard ELISA was performed to determine the levels of TNF-α and IL-6.
