Vitamin C Mitigates Oxidative Stress and Tumor Necrosis Factor-Alpha in Severe Community-Acquired Pneumonia and LPS-Induced Macrophages
Figure 4
The effect of vitamin C on oxidative stress and DNA damage in LPS-induced MH-S cells. ROS and DNA damage in MH-S cells were measured in the presence or absence of vitamin C and treated with LPS for 12 hours. (a) ROS were detected by confocal microscope using DCFH-DA probe. ROS level was represented by fluorescence intensity. (b) DNA damage was measured by comet assay. DNA damage score was analyzed with comet assay IV software. (c) MH-S cells viability was detected by confocal microscope using Edu staining (×200). Arrow showed viability of cells. (d) TNF-α was measured by ELISA. (e) TNF-α, P38, and p-P38 were determined by western blotting. β-actin was used as the loading control.
