Proteomic Analysis of Lung Tissue in a Rat Acute Lung Injury Model: Identification of PRDX1 as a Promoter of Inflammation
Figure 5
PRDX1 expression modulated inflammation in airway epithelium cells. (a) Overexpression of PRDX1 in BEAS-2B cells was analyzed by WB. β-Actin was used as a loading control. (b) Knockdown of PRDX1 in BEAS-2B cells was analyzed by WB. β-Actin was used as a loading control. Mock: cells were transfected with transfection reagent alone; shRNA-NC: cells were transfected with a shRNA vector. (c) Overexpression of PRDX1 increased the expression of proinflammatory cytokines. RT-PCR analysis of expression of IL-6, IL-8, and TNF-α in vector or pMSCV-PRDX1 transduced BEAS-2B cells treated with LPS or control. (d) Knockdown of PRDX1 inhibited the expression of proinflammatory cytokines. RT-PCR analysis of expression of IL-6, IL-8, and TNF-α in pSUPER-shRNA NC or pSUPER-shPRDX1#3 treated with LPS or control. (e) Expression of p65 in nuclear extracts of indicated cells was analyzed by western blotting. p85 was used as a loading control. Experiments were repeated at least 3 times, with similar results. Each bar represents the mean ± SD of three independent experiments..
