Interleukin-1 Receptors Are Differentially Expressed in Normal and Psoriatic T Cells
Figure 1
Distribution of CD4+ T lymphocyte subpopulations is not different in healthy and psoriatic peripheral blood samples. CD4+ T cells were isolated from healthy and psoriatic donors and were activated for two days with CD3/CD28 beads in presence of IL-2; control cells were incubated without any treatment. Cells were stained with anti-CD45RO-FITC and anti-CD25-APC to identify T cell subpopulations such as naïve T (TN, CD45RO−CD25−), memory T (TM, CD45RO+CD25−), naïve regulatory T (TNreg, CD45RO−CD25+), and regulatory T cells (Treg, CD45RO+CD25+) (a). Anti-GARP-PE antibody was used instead of anti-CD25 to differentiate activated cells (b). Representative scatterplots from healthy samples are shown. Percentage of cells in each population was determined and shown as the mean of four independent samples from healthy (H) and psoriatic (P) peripheral blood (c). Control resting cells and activated cells are represented by black and white bars, respectively.
