Chemokine CXCL16 Expression Suppresses Migration and Invasiveness and Induces Apoptosis in Breast Cancer Cells
Figure 2
Overexpression of CXCL16 inhibited migration and invasion and promoted apoptosis, of MDA-MB-231 cells. MDA-MB-231 cells were infected with negative lentivirus (LV-NC) or recombinant CXCL16 lentivirus (LV-CXCL16). 120 h later cells were collected and migration, invasion, proliferation, and apoptosis assays were conducted, respectively. (a) Recombinant CXCL16 significantly upregulated total CXCL16 (a1) and TM-CXCL16 (a2). (b) and (c) Overexpression of CXCL16 led to a decrease in migration (b) and invasion (c) of MDA-MB-231 cells. compared with MDA-MB-231 or negative control (LV-NC). n.s.: nonsignificant compared between MDA-MB-231 or negative control (LV-NC). (d) Proliferation of MDA-MB-231 cells was not affected by CXCL16 overexpression. n.s.: nonsignificant compared between MDA-MB-231 and negative control (LV-NC). (e) CXCL16 expression promoted apoptosis of MDA-MB-231 cells (left) in conjunction with enhanced caspase-3 activity (right). compared with MDA-MB-231 or negative control (LV-NC). n.s.: nonsignificant compared between MDA-MB-231 and negative control (LV-NC).
