Upregulation of IL-21R in tenocytes in vitro. (a) The levels of mRNA () for IL-21R were determined by real-time PCR in explanted tenocytes in vitro following stimulation with IL-1, TNF, and in combination. Data are shown as the mean fold change ± SD of triplicate samples compared to no stimulated cells corrected to GAPDH and are representative of experiments using five individual donors of tendon explant tissue. (b) Immunostaining for IL-21R in explant tenocyte cultures stimulated as in (a) confirming changes at protein level.
