Figure 1: Identification of isolated muscle-derived cells. (a) The appearance of muscle-derived colony 6 days after isolation; (b and c) fluorescence microscopy. Desmin protein stained with Alexa 594 conjugated Ab (red) in undifferentiated MDCs (b) and in MDCs induced to myogenic differentiation for 3 days (c); (d) the presence of lipid accumulating cells within MDC population, red lipid droplets indicated with white arrowhead (Oil Red O staining), and cell nuclei stained with DAPI (blue). A multinucleated myotube can be observed in the same field of view (arrow). Scale bars in (a) 200 μm, (b and c) 50 μm, and (d) 20 μm.