Consequences of the Lack of CD73 and Prostatic Acid Phosphatase in the Lymphoid Organs

Figure 1

Nucleotidase activities of purified human PAP and recombinant human CD73. (a) The activities of hPAP and hCD73 were determined spectrophotometrically at neutral or acidic pH by using artificial substrate p-nitrophenylphosphate (pNPP). ND: nondetectable. Radio-TLC analysis of nucleotidase activities was also performed by incubating purified hPAP (b) and hCD73 (c) with 40 μM [³H]AMP at different pH without and with 5 μM AMPCP, as indicated. Enzymatic activities are expressed as μmoles of pNPP or [³H]AMP hydrolyzed per hour by one milligram of enzyme protein. .

(a)

(b)

(c)