Herbal Extract SH003 Suppresses Tumor Growth and Metastasis of MDA-MB-231 Breast Cancer Cells by Inhibiting STAT3-IL-6 Signaling
Figure 3
SH003 inhibits MDA-MB-231 growth and induces apoptosis. (a) Different breast cancer cells were seeded on 96-well plates and treated with each extract at different concentrations for 72 hours. Experiments were performed three times in sextuplicate. Representative data were presented as the means and standard deviations. Right triangles indicate the doses of each extract (0, 50, 100, 200, and 500 μg/mL), which was also marked with bars in different colors. (b) MDA-MB-231 cells were treated with 500 μg/mL of the each extract. Cells were stained with propidium iodide (PI, 50 μg/mL) at room temperature in the dark. PI-positive apoptotic cells were detected using FACSCalibur. . (c) MDA-MB-231 cells were treated with the indicatives at 500 μg/mL for 24 hours and then subjected to western blots. Tubulin was used for the intimal control. (d) RIE cells were seeded on 96-well plates and treated with each extract at different concentrations for 72 hours. Experiments were performed three times in sextuplicate. Representative data were presented as the means and standard deviations.
