Research Article

Cholesterol Oxidase Binds TLR2 and Modulates Functional Responses of Human Macrophages

Figure 1

The effect of ChoD on TLR-2 and CR3 expression on THP-1-derived macrophages. TLR2 (a) and CR3 (b) expression on cells treated with ChoD (0.01–0.5 U/mL) for 24 h. Data are means ± SEM of MFI values from 5-6 separate experiments ( for macrophages + ChoD versus macrophages). (c) LTA (100 μg/mL, 30 min) binding to macrophages pretreated with ChoD (0.5 U/mL) for 24 h. After treatment with ChoD and LTA, macrophages were incubated with anti-LTA antibodies and secondary FITC-conjugated antibodies and then analyzed using flow cytometry. Data are expressed as means ± SEM of MFI values from seven independent experiments ( for macrophages + ChoD + LTA versus macrophages + LTA). Percentage values relative to control (100%) are shown on the right axis.