Lycopene Modulates THP1 and Caco2 Cells Inflammatory State through Transcriptional and Nontranscriptional Processes

Figure 3

Modulation by lycopene of reporter gene activity in THP1 cells. THP1 cells were transfected using one of the luciferase reporter genes controlled by the promoter/enhancer sequence derived from either the IgK gene (pGL2-IgK-luc) (a) or the IL-8 gene (pGL2-IL-8-luc) (b). The plasmid pGL2-AP1-luc (c) contains the AP1 specific sequence. After 12 h transfection, cells were washed and fresh medium with or without lycopene at the indicated concentration was added, and culture continued for 2 h. Then, according to assays, TNFα ((a), (b)) or PMA (c) was added and culture was resumed for 6 h. Cells were harvested, lysed, and placed at −80°C. Luciferase activity was recorded. Values are means ± SD (𝑛=8). Assays treated with lycopene were compared to corresponding assays without lycopene. ^∗∗∗𝑃<0.01; ^∗∗𝑃<0.05; ∗, nonsignificantly different.

(a)

(b)

(c)