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Mediators of Inflammation
Volume 2014 (2014), Article ID 510846, 9 pages
Research Article

The Relationship between the Antitumor Effect of the IL-12 Gene Therapy and the Expression of Th1 Cytokines in an HPV16-Positive Murine Tumor Model

1Division Chronic Infection and Cancer, Research Center in Infections Diseases, National Institute of Public Health, Avenida Universidad 655, Col. Santa María Ahuacatitlán, 62100 Cuernavaca, MOR, Mexico
2Institute of Immunology and Virology, School of Biological Sciences, Autonomous University of Nuevo Leon, Avenida Pedro de Alba y Manuel Barragán, 66450 Monterrey, NL, Mexico

Received 9 December 2013; Revised 20 February 2014; Accepted 6 March 2014; Published 7 April 2014

Academic Editor: Chiou-Feng Lin

Copyright © 2014 Flor García Paz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. The goal of the present study was to investigate the effect of IL-12 expressed in plasmid on the Th1 cytokine profile in an experimental HPV16-positive murine tumor model and the association with the IL-12’s antitumor effect. Methods. Mice were injected with BMK-16/myc cells to establish HPV16-positive tumor and then pNGVL3-mIL-12 plasmid; pcDNA3 plasmid or PBS was injected directly into tumor site. The antitumor effect of the treatment was evaluated and the cytokines expression profile in each tumor tissue was analyzed. Results. Treatment with pNGVL3-mIL-12 plasmid had a significant antitumor effect, and a Th2-Th3-type cytokines prolife was detected in the murine tumor model with expression of the cytokines IL-10, IL-4, and TGF-β1. However, after the tumor was treated with three intratumoral injections of plasmid containing IL-12 cDNA, it showed a cytokine profile associated with Th1 with expression of IL-2, IL-12, and IFN-γ cytokines and reduced expression of IL-10, IL-4, and TGF-β1. Conclusions. The treatment with the IL-12 gene in the experimental HPV16-positive tumor model promoted the activation of the cellular immune response via expression of a Th1-type cytokine profile and was associated with the inhibition of tumor growth. Thus, IL-12 treatment represents a novel approach for gene therapy against cervical cancer.