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Mediators of Inflammation
Volume 2014, Article ID 510846, 9 pages
Research Article

The Relationship between the Antitumor Effect of the IL-12 Gene Therapy and the Expression of Th1 Cytokines in an HPV16-Positive Murine Tumor Model

1Division Chronic Infection and Cancer, Research Center in Infections Diseases, National Institute of Public Health, Avenida Universidad 655, Col. Santa María Ahuacatitlán, 62100 Cuernavaca, MOR, Mexico
2Institute of Immunology and Virology, School of Biological Sciences, Autonomous University of Nuevo Leon, Avenida Pedro de Alba y Manuel Barragán, 66450 Monterrey, NL, Mexico

Received 9 December 2013; Revised 20 February 2014; Accepted 6 March 2014; Published 7 April 2014

Academic Editor: Chiou-Feng Lin

Copyright © 2014 Flor García Paz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Detection of cytokines expressed by BMK-16/myc cells. Five-microgram of total RNA extracted from BMK-16/myc cells were reversed transcribed and amplified by PCR. RT-PCR was performed, using specific primers for IFN-γ, IL-6, IL-4, TNF-α and TGF-β. PCR products were DNA were electrophoresed through 6% acrylamide gels and visualized with ethidium bromide staining. We used murine peripheral blood mononuclear cell activated with PHA as cytokines control. We detected that the BMK-16/myc cells expressed IL-4, TNF-α and TGF-β.

  1. Supplementary Material