Sphingosine-1-phosphate/S1P Receptors Signaling Modulates Cell Migration in Human Bone Marrow-Derived Mesenchymal Stem Cells
Figure 5
Involvement of the PTX-sensitive ERK1/2 pathway in S1P-induced migration of human BMSCs. (a), (c) Serum-starved BMSCs were pretreated with the indicated concentration of PTX (a) or U0126 (c) and then were allowed to migrate for 4 h in the presence of 1 nM S1P. (b) The effect of S1P on the activation of ERK1/2 MAPK. Cells were stimulated with S1P (1 μM) for the indicated time and cell lysates were analyzed by western blot. (d)-(e): The effect of S1PRs antagonists (S1PR1 antagonist W146, S1PR2 antagonist JTE-013, and S1PR3 antagonist CAY1444) and PTX on S1P-induced activation of ERK1/2. Cells were pretreated with S1PRs antagonists (d) or pretreated with 5 ng/mL PTX (e). The pretreated cells were stimulated with 1 μM S1P for 2 min, and cell lysates were analyzed by western blot. Data are presented as the mean ± SD. , compared with control.
