Regulation of Chemokine CCL5 Synthesis in Human Peritoneal Fibroblasts: A Key Role of IFN-γ
Figure 2
CCL5 induction in HPFB stimulated with TNF-α and IFN-γ. (a) Kinetics of CCL5 secretion by HPFB treated with TNF-α (1000 pg/mL) and IFN-γ (25 U/mL) alone or in combination. Asterisks represent a significant difference compared with the predictive additive values at each time point (); (b) dose effect of IFN-γ alone or with TNF-α (1000 pg/mL; ); (c) dose effect of TNF-α alone or with IFN-γ (25 U/mL; ). B and C cells were stimulated for 48 hours. Asterisks represent statistically significant differences compared to the predictive additive values; (d) kinetics of TNF-α and IFN-γ-induced CCL5 mRNA. Cells were treated with TNF-α and/or IFN-γ for the times indicated. Results of an exemplary experiment of two performed; (e) magnitude of CCL5 mRNA expression in HPFB treated for 24 hours with TNF-α and/or IFN-γ. Results of 4 experiments with cells from separate donors. Asterisks represent a significant difference compared to the predictive additive value. D and E cells were treated with TNF-α at 1000 pg/mL and IFN-γ at 25 U/mL. CCL5 mRNA expression relative to that of GAPDH was quantified with real-time PCR; (f) effect of neutralizing anti-TNF-α or anti-IFN-γ antibodies on synergistic CCL5 release by HPFB. Cells were incubated with antibodies (all at 1 μg/mL) for 48 h. Asterisks represent a significant difference compared with cells treated with a combination of TNF-α (1000 pg/mL) and IFN-γ (25 U/mL) in the absence of antibodies ().
