Preparation and Identification of Per a 5 as a Novel American Cockroach Allergen

<table>SDS-PAGE analysis of purification of Per a 5 in baculovirus-infected insect cells through Ni column. Lane M: protein standard; lane 1: elute with the elution buffer containing 250 mM imidazole; lane 2: elute with the elution buffer containing 20 mM imidazole; lane 3: elute with the elution buffer containing 50 mM imidazole; lane 4: run through; lane 5: the cytosol.</table>

Mediators of Inflammation

fig4

Figure 4

Figure 4: Preparation and Identification of Per a 5 as a Novel American Cockroach Allergen 