Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi

Figure 4

Frequencies of inflammatory cells in the hearts and skeletal muscles of resistant mice vaccinated by the heterologous prime-boost regimen. (a) F1 (CB10XBALB/c) female mice were immunized i.m., as depicted. Details on the dose are shown in Figure 3(a). (b) At 240 days, heart and skeletal muscle sections of mice challenged with the Brazil strain were stained with hematoxylin and eosin, and the number of inflammatory cells was quantified. Bars represent the mean of 6 mice/group ± SD. Asterisks denote that there was a significant difference between the indicated groups (^∗𝑃<0.05 or ^∗∗𝑃<0.01). (c) The same as above, except that the mice were challenged with parasites of the Colombian strain. Bars represent the mean of 5 mice/group ± SD.

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