Physical Exercise Reduces the Expression of RANTES and Its CCR5 Receptor in the Adipose Tissue of Obese Humans
Figure 3
Differential regulation of CCR5 in PBMCs and adipose tissue is associated with obesity. CCR5 gene expression was measured by qRT-PCR in PBMCs (a) from lean and obese nondiabetic subjects and in the adipose tissue (b) from lean and obese nondiabetic subjects. The data are presented as fold changes in obese compared to lean subjects after normalization with the GAPDH reference gene. (c) Characterization of the monocyte subpopulations and T cells in peripheral blood from lean and obese subjects. Monocytes subsets were defined by staining for CD14 (PE), T cells by CD3 (FITC), and expression of CCR5 (APC) and then were analyzed. Gates P4 and P3 define the CD14+ and CD14++ subsets, respectively. Left and right upper panels are representative dot plots of CD3 and CCR5 expression on T cells from lean and obese subjects, respectively. Left and right lower panels are representative dot plots of CD14 and RANTES expression on monocyte subsets from lean and obese participants, respectively. The double-positive populations (i.e., CD3+CCR5+, CD14+CCR5+, and CD14++CCR5+) were analyzed for mean CCR5 fluorescence intensity. For each experiment, the sample size from each group is indicated by .
