Physical Exercise Reduces the Expression of RANTES and Its CCR5 Receptor in the Adipose Tissue of Obese Humans
Figure 4
Physical exercise reduces the expression of RANTES and CCR5 in the adipose tissue. (a) IHC staining with RANTES antibody using subcutaneous adipose tissues from obese subjects before and after 3 months of physical exercise. Aperio software was used to quantify positive staining in obese before and after physical exercise. The data are plotted in a bar graph on the right of the figure as fold changes of RANTES protein expression in obese subjects before and after the physical exercise program. (b) qRT-PCR analysis of RANTES, CCR5, TNF-α, and IL-6 mRNA expression in the adipose tissue from obese before and after 3 months of physical exercise. (c) Graphic representation of IHC staining with TNF-α, anti-IL-6, and anti-Phospho-JNK antibodies using subcutaneous adipose tissues from obese subjects before and after 3 months of physical exercise. Aperio software was used to quantify positive staining in obese before and after physical exercise. Paired t-test for two group analysis was done to compare the expression of proteins and mRNA in obese before and after exercise. For each experiment, the sample size from each group is indicated by .
