GSK-3β Inhibition Attenuates CLP-Induced Liver Injury by Reducing Inflammation and Hepatic Cell Apoptosis

Figure 5

GSK-3β inhibition reduces the proinflammatory cytokine expression in cultured macrophages stimulated by LPS. (a) RAW264.7 cells were stimulated with LPS for 6 h in the absence or presence of SB216763 (10 μM). TNF-α, IL-6, IL-1β, and IL-10 mRNA expression levels were measured by quantitative PCR. (b) TNF-α and IL-6 concentration in the cultured medium was measured by ELISA. (c) RAW264.7 cells were stimulated with LPS for 1 h in the absence or presence of SB216763 (10 μM). NF-κB activity, as well as CREB activity, was assessed. Data are shown as mean ± SD. per group. , ^# compared with vehicle-treated group.

(a)

(b)

(c)