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Mediators of Inflammation
Volume 2014 (2014), Article ID 703653, 12 pages
Research Article

Monocyte Subsets in Schistosomiasis Patients with Periportal Fibrosis

1Serviço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40110-160 Salvador, BA, Brazil
2Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), CNPQ/MCT, Brazil
3Escola Bahiana de Medicina e Saúde Pública, 40050-420 Salvador, BA, Brazil
4Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, UFBA, 40170-115 Salvador, BA, Brazil

Received 25 September 2013; Revised 16 January 2014; Accepted 30 January 2014; Published 13 March 2014

Academic Editor: Dennis Daniel Taub

Copyright © 2014 Jamille Souza Fernandes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A major issue with Schistosoma mansoni infection is the development of periportal fibrosis, which is predominantly caused by the host immune response to egg antigens. Experimental studies have pointed to the participation of monocytes in the pathogenesis of liver fibrosis. The aim of this study was to characterize the subsets of monocytes in individuals with different degrees of periportal fibrosis secondary to schistosomiasis. Monocytes were classified into classical (CD14++CD16), intermediate (CD14++CD16+), and nonclassical (CD14+CD16++). The expressions of monocyte markers and cytokines were assessed using flow cytometry. The frequency of classical monocytes was higher than the other subsets. The expression of HLA-DR, IL-6, TNF-α, and TGF-β was higher in monocytes from individuals with moderate to severe fibrosis as compared to other groups. Although no differences were observed in receptors expression (IL-4R and IL-10R) between groups of patients, the expression of IL-12 was lower in monocytes from individuals with moderate to severe fibrosis, suggesting a protective role of this cytokine in the development of fibrosis. Our data support the hypothesis that the three different monocyte populations participate in the immunopathogenesis of periportal fibrosis, since they express high levels of proinflammatory and profibrotic cytokines and low levels of regulatory markers.