Immunomodulatory Effects of Polysaccharide from Marine Fungus Phoma herbarum YS4108 on T Cells and Dendritic Cells

Figure 2

The effect of YCP on DCs. (a) The expression of CD11c on DCs after 7-day generation with GM-CSF and IL-4. (b) The percentage of mature DCs (CD11c⁺/MHC II⁺) stimulated by Ag and YCP at the indicated concentrations. ((c) and (d)) CD80 on dendritic cells at different times (6 h, 12 h, 24 h, and 48 h) after the stimulations with medium/Ag/YCP+Ag/LPS+Ag. ((e) and (f)) CD86 on dendritic cells at different times (6 h, 12 h, 24 h, and 48 h) after the stimulations with medium/Ag/YCP+Ag/LPS+Ag. (g) Effect of YCP on DC IL-12 production. DCs were treated with Ag or YCP+Ag or LPS at the indicated concentrations, and IL-12 production was measured by ELISA assay at 48 h (, ^*, ^** versus medium by Kruskal-Wallis test followed by Dunn’s post hoc test. ^## versus medium by Mann-Whitney test).

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