Polymorphisms in the Tumor Necrosis Factor Receptor Genes Affect the Expression Levels of Membrane-Bound Type I and Type II Receptors

Table 1

Primers and restriction endonucleases used for SNP genotyping.

SNP	Primers	Sequence (5′ to 3′)	Restriction endonucleases
TNFRI − 609 G/T, rs4149570	Forward; Reverse	CGGACGCTTATCTAT ATCTC; TTGTAGTCCAGTCACAAGCA	Bst4C I
TNFRI − 1207 C/G, rs4149569	Forward; Reverse	TTGGGAGATGTCTGCATCAA; TTCTTCGTTTGCTTGTTTTTCA	BstC8 I
TNFRII − 1709 A/T, rs652625	Forward; Reverse	GAGTGCTGAGTGAGAAACTG; AGCTTGAATTCGTTCCCAGG	DseD I
TNFRII − 3609 C/T, rs590368	Forward; Reverse	ATGCTTTTGTCCATGCAGGT; GCTGTACCCCGTATTAGCTG	Msp I

SNP: single nucleotide polymorphism; TNFR: tumor necrosis factor receptor.