Overexpression of Receptor for Advanced Glycation End Products and High-Mobility Group Box 1 in Human Dental Pulp Inflammation
Figure 5
Inhibition of NF-κB activation attenuated LPS-induced HMGB1 translocation from the nucleus to the cytoplasm and release in OLC-1. Cells were pretreated with the selective inhibitor of human IKK-2 (TPCA-1), 2 h before the cells were stimulated with LPS for 24 h. (a) The cells were harvested, and the cytoplasmic proteins were isolated for analysis of HMGB1 by western blotting. A representative blot using antibodies against HMGB1 and β-actin is shown. HMGB1 expression was quantified by densitometry and normalized to β-actin. (b) HMGB1 release into the supernatant was analyzed by ELISA. Results are expressed as the means SD from triplicate experiments. .
