The Effects of Amphiregulin Induced MMP-13 Production in Human Osteoarthritis Synovial Fibroblast
Figure 5
AREG induced NF-κB activation through EGFR/PI3K/Akt pathway. ((a), (b), and (c)) OASFs were incubated with various concentrations of AREG or pretreated with PI3K inhibitors (LY294002 (5 μM) and Wortmannin (0.5 μM)), Akt inhibitor (Akti (5 μM)), or NF-κB inhibitors (PDTC (5 μM), or TPCK (5 μM)) for 30 min or transfected with PI3K, Akt, IKKα, and IKKβ mutant before exposure to AREG. NF-κB luciferase activity was measured, and the results were normalized to the β-galactosidase activity. (d) OASFs were pretreated with LY294002, Wortmannin, or Akti for 30 min and then stimulated with AREG for 120 min, and p65 immunofluorescence staining was examined. (e) OASFs were pretreated with LY294002, Wortmannin, or Akti for 30 min followed by stimulation with AREG for 60 min, and p-p65 expression was examined by Western blot analysis. (f) Schematic presentation of the signaling pathways is involved in AREG-induced MMP-13 production in human synovial fibroblast. Results are expressed as the mean ± SEM . compared with control; compared with AREG-treated group.
