Potent Anti-Inflammatory Activity of Pyrenocine A Isolated from the Marine-Derived Fungus Penicillium paxilli Ma(G)K
Figure 3
Anti-inflammatory effects of pyrenocine A on the production of TNF-α. (a) Pretreatment procedure: cells were pretreated with different concentrations of pyrenocine A (3.75–0.11 μM) for 2 h and then stimulated with LPS (1 μg/mL) during 18 h. (b) Posttreatment procedure: cells were stimulated with LPS (1 μg/mL) for 2 h and then pyrenocine A (3.75–0.11 μM) was added during 18 h. The supernatants were collected, and cytokine quantification was performed using an ELISA system. Results correspond to the mean ± SEM of six independent experiments performed in quadruplicate for each condition. * versus medium (control), # versus LPS (ANOVA followed by Bonferroni). ND = not detectable.
