Research Article

Impaired Resolution of Inflammation in the Endoglin Heterozygous Mouse Model of Chronic Colitis

Figure 3

Higher number of infiltrating myeloid cells in colonic lamina propria of colitic Eng +/− mice. (a) Representative flow cytometry contour plots of colonic lamina propria cells, isolated at day 0 and day 22 of a DSS-induced colitis experiment, show the distribution of CD11b+F4/80+ and CD11b+F4/80 cells. All plots were first gated for CD45+CD11b+ cells (not shown) and the percentage of CD45+ leukocytes is indicated. (b) The distribution and (c) total number of CD11b+F4/80+ cells in Eng +/+ and Eng +/− mice at days 0, 7–9, and 18–23. (d) Representative flow cytometry contour plots of colonic lamina propria cells isolated from both groups of mice at days 8 and 18 of the colitis course. Cells were initially gated for CD11b+F4/80 cells and analyzed for neutrophils (Ly6C+/Ly6G+) and monocytes (Ly6C+Ly6G). Histograms in (e) and (f) show the number of neutrophils and monocytes per mouse, respectively. Results represent mean ± SEM ( experiments for day 0, 5 for days 7–9, and 4 for days 18–23, with 3-4 mice/experiment). versus corresponding day 0.
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