Mediators of Inflammation

Mediators of Inflammation / 2014 / Article

Review Article | Open Access

Volume 2014 |Article ID 805841 | https://doi.org/10.1155/2014/805841

Elsayed A. Elsayed, Hesham El Enshasy, Mohammad A. M. Wadaan, Ramlan Aziz, "Mushrooms: A Potential Natural Source of Anti-Inflammatory Compounds for Medical Applications", Mediators of Inflammation, vol. 2014, Article ID 805841, 15 pages, 2014. https://doi.org/10.1155/2014/805841

Mushrooms: A Potential Natural Source of Anti-Inflammatory Compounds for Medical Applications

Academic Editor: Julio Galvez
Received24 May 2014
Accepted13 Aug 2014
Published23 Nov 2014

Abstract

For centuries, macrofungi have been used as food and medicine in different parts of the world. This is mainly attributed to their nutritional value as a potential source of carbohydrates, proteins, amino acids, and minerals. In addition, they also include many bioactive metabolites which make mushrooms and truffles common components in folk medicine, especially in Africa, the Middle East, China, and Japan. The reported medicinal effects of mushrooms include anti-inflammatory effects, with anti-inflammatory compounds of mushrooms comprising a highly diversified group in terms of their chemical structure. They include polysaccharides, terpenoids, phenolic compounds, and many other low molecular weight molecules. The aims of this review are to report the different types of bioactive metabolites and their relevant producers, as well as the different mechanisms of action of mushroom compounds as potent anti-inflammatory agents.

1. Introduction

In the human body, inflammation is considered to be part of the complex biological response to remove injury or harmful stimuli such as pathogens, damaged cells, or irritation. This response leads to many physical symptoms such as fever, pain, and swelling, as a result of many associated changes such as vasodilation, increased vascular permeability, and plasma extravasation. Nowadays, the nonsteroidal anti-inflammatory drugs (NSAIDs) are usually the most commonly administrated drug to reduce inflammation in the body. Many studies, however, have shown that the long-term administration of NSAIDs has the potential for significant side effects on the gastrointestinal tract (GIT). These include numerous harmful effects such as mucosal lesions, bleeding, peptic ulcers, and intestinal perforation [1, 2]. Other studies, meanwhile, have suggested that the side effects of NSAIDs are not limited only to GIT but extend to other serious complications such as acute renal failure, nephrotic syndrome, hypertension, and cardiovascular toxicity [35]. Recently, therefore, much effort has been devoted towards the discovery of alternative anti-inflammatory compounds of plant origin as potential natural and safe medicines without the harmful side effects of NSAIDs [611]. While a variety of plants have traditionally been used in human medicine, mushrooms also have a long history as important components of folk medicine and have been widely used in the form of aqueous extracts in many African, Middle Eastern, European, Asian, and native Australian cultures for the treatment of different diseases as well as a preventive medicine [1216].

Mushrooms are a very large and diversified group of macrofungi belonging to Basidiomycetes and Ascomycetes; with a cell cycle including the formation of sexual spores. The fungal spores for these two groups are located in a special structure called the basidium (for Basidiomycetes) or the ascus (for Ascomycetes), and such mushrooms can grow either above the earth (epigeous macrofungi), giving mainly umbrella like structures which include basidiospores, or at depths of 10–20 cm below the soil surface (hypogeous macrofungi or truffles). The latter of these belong mainly to Ascomycetes and usually grow in a symbiotic relationship with a host plant as ectomycorrhizal mushrooms (EMM).

Both types of mushrooms have considerable nutritional value, since they are rich sources of carbohydrates, proteins, free amino acids, and vitamins, as well as different essential minerals and trace elements [17, 18]. They are also rich in many bioactive metabolites of high medicinal value such as lectins, polysaccharides, phenolics and polyphenolics, terpenoids, ergosterols, and volatile organic compounds [1921]. Mushroom extracts have therefore been used medicinally in immunomodulator [2225], antitumor/anticancer [2630], antibacterial and antiviral [3133], antioxidant [3437], and antihypoglycaemic [3840] applications and as active medicines in the prevention of cardiovascular diseases through their action as antiatherosclerotic agents [41]. In addition, many compounds of highly diversified chemical structures with anti-inflammatory activities have been isolated and purified from different types of mushrooms. It has been reported, for example, that water, methanolic, ethanolic, and ethyl acetate extracts of different epigeous and hypogeous mushrooms showed significant decreases in the activities of inflammatory mediators such as nitric oxide (NO), cytokines, and prostaglandins, thus inhibiting some macrophage functions and reducing cell inflammations.

2. Inflammation and Cell Molecular Signaling

Inflammation is a complex set of interactions among soluble factors and cells that can arise in any tissue in response to trauma, infections, or postischaemic, toxic, or autoimmune injury [42]. In normal cases, the body’s response to inflammation is self-limiting through the downregulation of proinflammatory protein expression, the increased expression of anti-inflammatory proteins, and a reversal in the vascular changes that facilitated the initial immune cell recruitment process [43]. The processes leading to inflammation are usually linked to the activities of the cells involved in the restoration of tissue structure and function. When cells are exposed to immune stimulants, the proinflammatory cells, such as macrophages, monocytes, or other host cells, start to produce many molecular mediators which initiate the inflammation process. Of the various inflammatory biomarkers that are produced, the most well-known are interleukins (IL-1β), IL-6; IL-8; tumour necrosis factor (TNF-α); nuclear factor-κB (NF-κB), intercellular adhesion molecule-1 (ICAM-1), inducible type cyclooxygenase- (COX-) 2, prostaglandin E2 (PGE2); 5-lipooxygenase (5-LOX); and inducible nitric oxide synthase (iNOS), which leads to the production of reactive nitrogen species such as nitric oxide (NO). Overproduction of these inflammatory mediators leads to different kinds of cell damage. In addition, prolonged inflammation causes many inflammatory diseases such as juvenile idiopathic arthritis (JIA), inflammatory bowel disease (IBD), multiple sclerosis, rheumatoid arthritis, gastritis, bronchitis, and atherosclerosis [44]. A strong link has also been established between long-term inflammation and the development of cancers [45]. In some types of cancer, inflammation occurs before malignant changes in the cells. In other types, oncogenic change induces an inflammatory microenvironment that promotes tumour development [46]. As a result, increased attention is now being focused on efforts to discover bioactive compounds which have the ability to suppress the production of inflammatory mediators. In this context, mushroom metabolites have been employed as potent, natural, and safe anti-inflammatory compounds based on their ability to reduce the production of inflammatory mediators through downregulation of the gene expression of different types of these inflammatory mediators.

3. Anti-Inflammatory Compounds of Mushrooms and Modes of Action

Mushrooms are widely used for their high nutritional value as a functional food. Additionally, they have been highly appreciated for their medicinal and therapeutic applications [47, 48]. Edible mushrooms produce a vast diversity of bioactive compounds such as polysaccharides, proteoglucans, terpenoids, phenolic compounds, steroids, and lectins. These compounds have a wide range of therapeutic effects and can act as immune-modulatory, anticarcinogenic, antiviral, antioxidant, and anti-inflammatory agents [16, 20]. The concentration and efficacy of the bioactive compounds are varied and depend on the type of mushroom, substrate applied, cultivation and fruiting conditions, stage of development, age of the fresh mushroom, storage conditions, and processing and cooking procedures [41]. Many of the bioactive compounds found in mushrooms exhibit significant anti-inflammatory properties. Table 1 lists most of the well-known mushroom species reported in the literature as possessing anti-inflammatory activities, along with their bioactive compounds and the solvent applied for extraction.


Mushroom speciesBiomaterial source Extracting solvent Bioactive compound References

Agaricus blazei WM1Polysaccharides[51]
A. bisporus WM3Pyrogallol, hydroxybenzoic acid derivatives, flavonoids.[91]
FB4Polysaccharide (fucogalactan)[65]
A. subrufescens WM2Polysaccharide (proteoglucan)[55]
Agrocybe aegerita FB3Fatty acids [88]
A. cylindracea FB2Agrocybin[98]
Albatrellus caeruleoporus FB3Phenolic compound (Grifolinones A, B) [93, 94]
Amanita muscaria FB 2, 3, 4Crude extract[99]
Boletus edulis WM 3polysaccharides, [91]
Cantharellus cibarius WM3Pyrogallol, flavonoids, polysaccharides [91]
C. tubaeformis WM2Polysaccharides[74]
Cordyceps militaris SC/FB4Crude extract [100]
C. pruinosa FB3Crude extract [101]
Caripia montagnei FB 3Polysaccharides (Glucans)[63]
Cyathus africanus SC5Diterpenoid[76]
C. hookeri SC5Diterpenoid [77]
Daldinia childiae FBN.M.Benzophenones (daldinals A-C)[92]
Elaphomyces granulatus FB4Syringaldehyde, syringic acid[19, 96]
Flammulina velutipes WM4Polysaccharides [72]
Fomitopsis pinicola SC4Polysaccharides[102]
Grifola frondosa SC5, 6Agaricoglycerides [97]
SC3, 5, Ergosterol (1-oleoyl-2-linoleoyl-3-palmitoylglycerol)[103]
Ganoderma lucidum FB4Triterpene[78]
FB 4Lucidenic acid, ganoderic acid [81]
Geastrum saccatum FB4Polysaccharides (β-glucans)[104]
FB2, 4, 5Crude extract [82]
Inonotus obliquus FB8, 5Ergosterols, lanosterol, inotodiol, trametenolic acid [84]
FB3Crude extract [83]
FB4Crude extract [105]
Lactarius deliciosus WM3Pyrogallol, flavonoids[91]
L. rufus FB2, 4Polysaccharides: (13), (16)-β-D-glucans[64]
Lentinus edodes FB2Heterogalactan (fucomannogalactan) with main chain of (1→6)-linked α-D-glactopyranosyl unit [66]
L. polychrous SC 4 Crude extract [67]
Lyophyllum decastes FB3Polysaccharides: (1–3) and (1–6) β-D-glucans [106]
Phellinus linteus FB9Polysaccharides (proteoglycan)[69]
FB4, 7, 9Crude extract[107]
Pholiota nameko WM4, 6, 10Polysaccharides [71]
Pleurotus pulmonarius FBN.M.Polysaccharides (1→3), (1→6)-linked β-glucan[60]
FB2, 4Polysaccharides (Glucan)[57]
Poria cocos SC 4Polysaccharides [108]
Termitomyces albuminosus SC/FB4Crude polysaccharide extract [68]

WM, whole mushroom; SC, submerged culture; FB, fruiting bodies.
Solvent: N.M., not mentioned; 1, chloroform; 2, water; 3, methanol; 4, ethanol; 5, ethyl acetate; 6, acetone; 7, n-hexane; 8, petroleum ether; 9, n-butanol; 10, acetyl ether.
3.1. Polysaccharides

Polysaccharides represent the major class of bioactive compounds found in mushrooms, and have been reported in most of the edible types. Bernardshaw et al. [49] investigated the effect of an aqueous polysaccharide extract of Agaricus blazei Murill on the stimulation of proinflammatory cytokine production in human monocytes and human vein endothelial cells in vitro. They reported that A. blazei extract is rich in β(1,3)-, β(1,4)-, and β(1,6)-D-glucans (Figure 1) and induces the release of proinflammatory cytokines (IL-1β, IL-6, IL-8) and αTNF. Another research has also reported that A. blazei extract enhances local and systemic inflammation, upregulates proinflammatory molecules, and enhances leukocyte homing to atherosclerosis sites without affecting the lipoprotein profile [50].

Contrary to these results, reports have recently appeared indicating that polysaccharides from A. blazei exert anti-inflammatory activities. Song et al. [51] investigated the anti-inflammatory and antiallergic effects of the chloroform-soluble extract of A. blazei in mouse bone-marrow-derived mast cells (BMMCs). They found that the extract inhibited the production of IL-6 in phorbol myristate acetate (PMA) plus calcium ionophore A23187-stimulated BMMCs and downregulated the phosphorylation of the serine/threonine kinase AKT. Furthermore, the extract inhibited the degranulation of β-hexosaminidase, as well as the production of prostaglandin D(2) and leukotriene C(4). They concluded that their A. blazei extract has anti-inflammatory and antiallergic activities, which are regulated by affecting IL-6, prostaglandin D(2), leukotriene C(4), and the phosphorylation of the serine/threonine kinase AKT.

In 2009, Johnson et al. [52] reported a phase I clinical trial in which 15 healthy individuals were given a daily oral dose of 60 mL of aqueous extract of AndoSan (a mushroom extract mixture containing 82% A. blazei mycelium, 15% Hericium erinaceum, and 3% Grifola frondosa) for 12 days. Their results showed a significant in vivo reduction in the levels of IL-1β, TNF-α, IL-6, IL-2, and IL-7 proinflammatory cytokines and unaltered levels of the remaining 12 cytokines. They concluded that the applied extract has an anti-inflammatory effect [52]. In 2011, they conducted another clinical pilot study in order to prove these results. They treated 21 hospitalized patients with inflammatory bowel diseases, ulcerative colitis, and Crohn’s disease with the same AndoSan treatment dose (60 mL/d for 12 days). They noticed a significant decrease in plasma levels of the proinflammatory cytokines as well as a decrease in calprotectin, an inflammatory marker, in the faeces of ulcerative colitis patients [53].

Ellertsen and Hetland [54] attributed the apparently contradictory findings reported above to the fact that the effect of A. blazei extract differs according to the molecular size of active compound and the type of study performed (i.e., in vitro or in vivo). They found that in vitro cell culture studies showed increased proinflammatory cytokines, while in vivo human studies showed an anti-inflammatory response. They suggested that cells in vitro are affected by all substances present in the extract, including high molecular weight β-glucans. On the other hand, in human in vivo studies, it is mainly the smaller substances which are absorbed into the digestive tract to become active in blood. Additionally, they found that the regulatory genes of the leukocytes affected by the A. blazei extract differ in their expression in vitro and in vivo. Genes regulating proinflammatory cytokines were strongly induced in vitro, probably by the high molecular weight β-glucans, whereas, in vivo, genes controlling anti-inflammatory reactions were upregulated, due to low molecular weight glucans. This hypothesis was supported by the mixed composition of the AndoSan extract, which contained both high and low molecular weight polysaccharides.

Other studies have reported that the protein-bound polysaccharides in the aqueous extract of A. subrufescens enhance defence mechanisms against invasive organisms and bacterial infection and reduce the plaque formation of viruses in cell cultures [49, 55, 56].

Additionally, glucans ((1→3)-β-D-glucopyranosyl) from Pleurotus pulmonarius have been reported to exhibit anti-inflammatory properties [5759]. These studies demonstrated that the fruiting body, or mycelial extract, of the edible mushroom markedly attenuated or suppressed symptoms associated with dextran sulphate sodium-induced acute colitis in mice. They also reported that, in an in vitro study, these glucans inhibited TNF-α-dependent activation of NF-κB in human intestinal cells [57]. Furthermore, β-glucans from P. pulmonarius exhibited an anti-inflammatory response in a model of acute colitis in rats, and when those from P. ostreatus were tested, they inhibited leukocyte migration to acetic acid-injured tissues [60, 61]. Recently, Jedinak et al. [62] showed that oyster mushroom concentrate (OMC) containing α- and β-glucans suppressed LPS-induced dependent activation of TNF-α, IL-6, and IL-12 in RAW 264.7 murine macrophage cells, as well as inhibiting LPS-induced production of prostaglandin E2 (PGE2) and nitric oxide (NO). This was mainly attributed to the downregulation of COX-2 and iNOS expression, respectively. They also reported that OMC significantly suppressed LPS-induced production of TNF-α in mice and concanavalin A-stimulated proliferation and secretion of INF-γ, IL-2, and IL-6 in mouse splenocytes [62].

The methanolic extract of Caripia montagnei (mainly polysaccharides of the glucan type) has also been shown to exert anti-inflammatory effects on male Swiss mice and male Wistar rats. The glucans significantly reduced the inflammatory infiltrate produced by thioglycolate-mediated peritonitis by about 75%. Additionally, a significant reduction in the nitric oxide levels was observed in the exudates [63]. Ruthes et al. [64], meanwhile, investigated water and ethanolic extracts of (1→3), (1→6)-β-D-glucans from the fruiting bodies of Lactarius rufus for their anti-inflammatory effects in both male and female Swiss mice. They found that soluble glucans inhibit inflammatory pain caused by formalin in comparison to the insoluble glucans, concluding that solubility and/or branching degree can alter the activity of β-glucans.

Furthermore, the aqueous extract of fucogalactan from Agaricus bisporus exhibited anti-inflammatory response in male Swiss mice [65]. It inhibited the neurogenic and inflammatory phases of formalin-induced licking, which they attributed to the decreased iNOS and COX-2 expression. Additionally, heterogalactans isolated by cold aqueous extraction from Lentinus edodes have shown anti-inflammatory activities in male Swiss mice [66]. The active fraction was made of fucomannogalactan with a main chain of (1→6)-linked α-D-galactopyranosyl units, partially substituted at O-2 with single-unit β-D-Manp or α-L-Fucp side chains. The polysaccharide produced a marked effect against acetic acid induced visceral nociception and inhibited the peritoneal capillary permeability and leukocyte infiltration.

Mushroom polysaccharides with anti-inflammatory properties have been also reported in crude extracts of Lentinus polychrous, Termitomyces albuminosus, and Phellinus linteus. In L. polychrous, they inhibited NO and proinflammatory productions by downregulating the gene expressions of proinflammatory mediators, thereby decreasing paw oedema in rats [67]. In T. albuminosus, they decreased the acetic acid induced writhing response and the licking time in the late phase in the formalin test in male ICR mice [68]. Additionally, mouse ear swelling was inhibited by 61.8, 79.0, and 81.6% when the animals were treated with the dry matter of the culture broth (1000 mg/kg), crude saponin extract (200 mg/kg), or crude polysaccharide extract (200 mg/kg), respectively. In another study, polysaccharides of P. linteus inhibited the mouse ear oedema induced by croton oil, reduced the amount of writhing induced by acetic acid in mice [69], induced heme oxygenase-1 (HO-1) of the RAW 264.7 macrophages, and suppressed iNOS and the subsequent production of nitric oxide by downregulation of iNOS promoter activity in lipopolysaccharide-stimulated macrophages [70].

The polysaccharides of Pholiota nameko have been reported to inhibit topical oedema in mouse ears and significantly suppress the development of egg albumin-, carrageenan-, and formaldehyde-induced paw oedema in the animals, and, significantly, did not produce any gastric lesions in rats [71]. The polysaccharides of the golden needle mushroom (Flammulina velutipes) are composed of three monosaccharides (glucose, mannose, and xylose) in a molar ratio of 3.5 : 0.8 : 1.4 and have been found to have anti-inflammatory activities and significantly decreased CD4+ CD8+, ICAM-1, and MPO in both the serum and colon of normal and burned rats [72]. Tibetan mushroom polysaccharides also exhibited an anti-inflammatory activity by significantly inhibiting the formation of granuloma tissue by about 43% as well as significantly decreasing rat paw oedema induced by carrageenan [73]. Polysaccharides extracted from Cantharellus tubaeformis [74] and Lactarius flavidulus [75] have also been reported to exhibit anti-inflammatory properties. Table 2 summarizes some of the biological studies carried out with different compounds having anti-inflammatory activities isolated from mushrooms.


Bioactive compound/mushroom speciesAssay modelResults/mechanism of actionReferences

Polysaccharides
 Agaricus blazei (i) Mouse bone marrow-derived mast cells (BMMCs) stimulated with PMA + A23187
(i) Inhibition of IL-6 production, downregulation of phosphorylation of Akt, inhibition of -hexosaminidase degranulation, inhibition of prostaglandin D(2), and leukotriene C(4) production.
[51]
(i) Male Swiss mice (acetic acid induced inflammation)(i) Dose-dependent anti-inflammatory response, inhibition of leukocyte migration (82%), IC50 of 1.19 (0.74–1.92) mg/kg, 3 mg/kg i.p. glucan injection reduced 85% of writhes[60]
 Pleurotus pulmonarius (ii) Mice (3.5% dextran sulfate sodium, DSS in drinking water for 14 days, with 20 mg fruiting body or mycelia extract/mouse/day)(ii) Fruiting body and mycelia extracts suppressed inflammatory reactions in vivo in DSS induced colonic inflammation by downregulating TNF-α secretion and inhibiting NF-B activation[57, 58]
(iii) Acetic acid induced colitis in rats (2% pleuran, or 0.44% hydrogel for 4 weeks)(iii) Reduction in macroscopic damage score by 51 and 67% for pleuran diet and hydrogel, respectively; reduction in the activity of myeloperoxidase and neutrophil infiltration[61]
(iv) Murine macrophage RAW264.7 cells, female Balb/C mice
(iv) Suppression of LPS-induced dependent activation of TNF-, IL-6, and IL-12, inhibition of LPS-induced production of PGE2 and NO. Suppression of LPS-induced production of TNF- in mice and concanavalin A-stimulated proliferation and secretion of INF-, IL-2, and IL-6 in mouse splenocytes
[62]
 Caripia montagnei (i) Male Swiss mice treated with 10, 30, and 50 mg/kg with mushroom glucan
(i) 50 mg/kg glucan reduced inflammatory infiltrate produced by thioglycolate-induced peritonitis by 75.5%, reduced NO level, IL-1ra, IL-10, and IFN-
[63]
 Lactarius rufus (i) Swiss mice, formalin-induced nociception, 30 mg/kg i.p. of fruiting body extract (soluble, insoluble, and modified)
(i) Inhibition of neurogenic pain by 36, 47, and 58% for soluble, insoluble, and modified glucans, respectively
[64]
 A. bisporus (i) Male Swiss mice, formalin-induced licking
(i) Inhibition of neurogenic and inflammatory phases, antinociceptive effect with IC50 of 36.0 (25.8–50.3 mg/kg)
(ii) Decreased iNOS and COX2
[65]
 Lentinus edodes (i) Male Swiss mice, acetic acid induced inflammation, 3–100 mg/kg i.p. fruiting body concentrate
(i) Inhibition of induced nociception with IC50 of 13.8 (7.8–23.5) mg/kg, 97% inhibition at 100 mg/kg
(ii) Inhibition of peritoneal capillary permeability and leukocyte infiltration (76% inhibition), IC50 13.9, 8.2–23.7, and 100% inhibition, IC50 6.5, 1.5–28.2 mg/kg, respectively
[66]
 L. polychrous (i) Carrageenan-induced paw edema in male Sprague-Dawley rats, murine macrophage RAW 264.7 cells
(i) Dose-dependent inhibition of NO, intracellular production
(ii) Decreased expression of iNOS, IL-1, IL-6, TNF-α, and COX-2
[67]
 Termitomyces albuminosus (i) Acetic acid induced writhing in male ICR mice, formalin test, xylene, and carrageenan induced ear edema
(i) Inhibition of ear swelling by 61.8, 79.0, and 81.6% for treatment with dry matter of the culture broth (1000 mg/kg), crude saponin extract (200 mg/kg), or crude polysaccharide extract (200 mg/kg), respectively
[68]
 Phellinus linteus (i) Croton oil induced ear edema and acetic acid induced writhing in male ICR mice
(i) Extract treatment with 1 mg/ear gave 45 and 41.5% inhibition in ear plug weight and thickness, respectively, oral administration of extract (100–400 mg/kg) inhibited writhing number (35.9–68.9%)
[69]
 Pholiota nameko (i) Xylene induced ear edema, adult Swiss mice and Sprague-Dawley rats, formaldehyde, egg albumin, and carrageenan induced paw edema in rats and mice
(i) Extract (5 mg/ear) inhibited ear edema, suppression of egg albumin, carrageenan and formaldehyde-induced paw edema at 100–400 mg/kg i.p., 10.96–43.75% inhibition of granuloma tissue growth, no production of gastric lesions in rats
[71]
 Flammulina velutipes (i) Male Wistar rats, fed 100–300 mg/kg mushroom for 30 days(i) Decreased levels of CD4+ CD8+, MPO, and ICAM-1, with increased level in IL-10 in serum[72]

Terpenoids
 Cyathus africanus (i) Mouse monocyte-macrophage RAW 264.7 cells, NO assay
(i) Cyathins D-H 3 and 5, neosarcodonin, and 11-O-acetylcyatha-triol inhibited NO production with an IC50 value of 2.75, 1.47, 12.0, and 10.73 M, respectively
[76]
 C. hookeri (i) Mouse monocyte-macrophage RAW 264.7 cells, NO assay
(i) Inhibition of NO production with an IC50 of 15.5, 52.3, and 16.8 M, respectively.
[77]
Ganoderma lucidum (i) LPS-stimulated murine macrophage RAW 264.7 cells, NO assay(i) Inhibition of TNF-, IL-6, NO, and PGE2, downregulation of iNOS and COX-2, inhibition of NF-B, decreased NF-B-DNA binding activity, and suppression of p65 phosphorylation[78]
(ii) Acetic acid induced ear edema in female ICR and SENCAR mice
(ii) Significant inhibition of inflammation (1 g/ear) in mice with IC50 values between 0.07 and 0.39 mg/ear, with inhibition ratio ranging from 58 to 97%
[81]
(i) Reduced nitrate levels by an average of 50%, dose-dependent inhibition of IL-1, IL-6, and TNF[82]


 Inonotus obliquus


(i) Murine macrophage RAW 164.7 cells
(ii) Trametenolic acid, ergosterol peroxide, 3-hydroxy-8,24-dien-21-al, ergosterol and inotodiol inhibited NO production, and NF-B luciferase activity, with an inhibition percentage of 50.04, 36.88, 20.36, 6.00, and 3.13%, respectively[84]
(iii) Methanolic extract inhibited production of NO, prostaglandin E2, and TNF-, inhibition of mRNA expression of iNOS and COX-2[83]

Peptides
 Cordyceps sinensis (i) Acetic acid induced inflammation in mice(i) Decreased level of TNF-, IL-1, dose-dependent inhibition of abdominal constrictions[86]

Phenolics
 Lactarius deliciosus (i) LPS-stimulated RAW 364.7 macrophage cells, nitrite, and cytokine assays
(i) 0.5 mg/mL mushroom extract inhibited NO production and expression of iNOS, IL-1, and IL6 mRNAs
[91]
 Daldinia childiae (i) LPS-stimulated RAW 264.7 macrophage cells
(i) Daldinals suppressed NO production with IC50 values ranging between 4.6 and 15.2 M and inhibited iNOS mRNA synthesis
[72]
 Albatrellus caeruleoporus (i) LPS-stimulated mouse macrophage RAW 264.7 cells(i) Grifolins inhibited NO production with IC50 values ranging between 22.9 and 29 M[94]

Syringaldehyde and syringic acid
 Elaphomyces granulates (i) Mouse macrophage RAW 264.7 cells(i) Crude ethanolic extract (50 g/mL) inhibited COX-2 activity by 68%, purified syringaldehyde, and syringic acid inhibited COX-2 activity in a dose-dependent manner, with an IC50 of 3.5 and 0.4 g/mL, respectively[96]

Agaricoglycerides
 Grifola frondosa (i) Acetic acid- and formalin-induced inflammation in Wister rats, treatment with orally fed extracts (100–500 mg/kg/day)(i) 500 mg/kg/day inhibited induced upregulation of NF-B and the production of IL-1, TNF-, ICAM-1, COX-2, and iNOS, suppressed acetic acid induced abdominal constrictions and formalin-induced spontaneous nociceptive behavior[97]

3.2. Terpenoids

Terpenes are the largest group of anti-inflammatory compounds in mushrooms and have been isolated from a range of different strains (Figure 2). Han et al. [76] isolated five novel cyathane diterpenes, identified as cyathins D-H (1–5), in addition to three well known diterpenes, namely, neosarcodonin, cyathatriol, and 11-O-acetylcyathatriol from the ethylacetate extract of Cyathus africans. They found that cyathins D-H 3 and 5, as well as neosarcodonin and 11-O-acetylcyathatriol, showed potent inhibition activity against NO production in lipopolysaccharide-mouse monocyte-activated macrophage RAW 264.7, with IC50 values of 2.75, 1.47, 12.0, and 10.73 μM, respectively. Xu et al. [77] isolated three bioactive diterpenes from the ethylactetate extract of C. hookeri Berk, namely, cyathin, (12R)-11a,14a-epoxy-13a,14b,15-trihydroxycyath-3-ene, and erinacine I. They also found that each of their three isolated diterpenes showed potent anti-inflammatory activities, inhibiting NO production in mouse monocyte-macrophages RAW 264.7, with an IC50 of 15.5, 52.3, and 16.8 μM, respectively.

Another class of terpenoids (triterpenes) have also been shown to exhibit high anti-inflammatory properties [78]. The authors of this study evaluated the anti-inflammatory effects of triterpene extract of Ganoderma lucidum in lipopolysaccharide- (LPS-) stimulated macrophages. They reported that the triterpene extract significantly suppressed the secretion of inflammatory cytokine tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6), as well as the inflammatory mediators nitric oxide (NO) and prostaglandin E2 (PGE2), from LPS-stimulated murine RAW 264.7 cells. Additionally, the LPS-dependent expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in RAW 264.7 cells were downregulated. The anti-inflammatory effects of the triterpene extract have been attributed to the inhibition of transcription factor NF-κB, as observed in the decreased NF-κB-DNA binding activity, as well as the suppression of p65 phosphorylation in the treated LPS-stimulated macrophages. Additionally, the extract inhibited LPS-dependent AP-1-DNA binding activity and downregulated the expression of AP-1 subunit c-Jun. Furthermore, the activity of MAP kinases has been suppressed, as observed by the downregulation of LPS-induced phosphorylation of ERK1/2 and JNK.

Furthermore, different lanostane-type triterpenic acids with potent anti-inflammatory properties have been isolated from G. lucidum [79, 80]. Akihisa et al. [81] reported that nine lucidenic acids and four ganoderic acids (Figure 3), isolated from the fruiting bodies of G. lucidum, significantly inhibited 12-O-tetradecanoylphorbol-13-acetate induced inflammation (1 μg/ear) in mice, with IC50 values between 0.07 and 0.39 mg/ear.

Different sterols (Figure 4) with potent anti-inflammatory activities have been also isolated and purified from the edible mushroom Inonotus obliquus. These significantly inhibited the levels of IL-1β, IL-6, and TNFα in a dose-dependent manner in murine macrophage RAW 164.7 cells [82]. Additionally, Park et al. [83] found that the methanolic extract of I. obliquus significantly inhibited NO production, prostaglandin E2, and TNF-α in LPS-stimulated RAW 264.7 macrophages. Ma et al. [84] isolated and identified six known triterpenes from the sclerotia of I. obliquus. They reported that trametenolic acid, ergosterol peroxide, 3β-hydroxy-8,24-dien-21-al, ergosterol, and inotodiol inhibited NO production and NF-κB luciferase activity in macrophage RAW 264.7 cells, with an inhibition percentage of 50.04, 36.88, 20.36, 6.00, and 3.13%, respectively.

3.3. Peptides

In addition to the previous bioactive compounds, anti-inflammatory peptides of different molecular weights have been isolated from mushrooms. Cordymin, a low molecular weight peptide (10,906 Da), has been purified from the medicinal mushroom Cordyceps sinensis [85, 86] and from C. militaris [87]. This peptide significantly inhibited the infiltration of polymorphonuclear cells and IR-induced upregulation of C3 protein produced in the brain, interleukin-1β, and tumour necrosis factor-α, which had a neuroprotective effect on the ischemic brain, due to the inhibition of inflammation. Agrocybin represents another peptide isolated from the edible mushroom Agrocybe cylindracea. Agrocybin exhibited antifungal activity against Mycosphaerella arachidicola, with an IC50 value of 125 μM at different temperatures up to 80°C [88].

3.4. Phenolics

Many edible mushrooms have been found to exhibit anti-inflammatory properties due to the presence of some phenolic compounds (Figure 5). For example, the common phenolic molecule pyrogallol has been extracted from Agaricus bisporus, Cantharellus cibarius, and Lactarius deliciosus [89, 90]. Pyrogallol inhibited NO production and iNOS, IL-1β, and IL6 mRNAs expression in response to LPS-stimulated RAW 364.7 macrophages [91]. Additionally, daldinals have been purified from Daldinia childiae. They strongly suppressed the LPS-induced production of NO in RAW 264.7 cells, with IC50 values ranging between 4.6 and 15.2 μM depending on the derivative [92]. This effect was attributed to the inhibition of iNOS mRNA synthesis.

Grifolin and grifolin derivatives (Figure 6) represent another class of farnesyl phenolic compounds which have been isolated from the edible mushroom Albatrellus ovinus and which exhibit anti-inflammatory properties [93]. Grifolins showed significant inhibition of NO production stimulated by LPS in RAW 264.7 cells, with IC50 values ranging between 22.9 and 29 μM [94], as well as inhibiting histamine release from rat peritoneal mast cells [95].

3.5. Miscellaneous

The ectomycorrhizal edible truffle Elaphomyces granulatus has been evaluated for its anti-inflammatory effects. The 95% ethanolic extract of the fruiting bodies contained two active aromatic compounds with anti-inflammatory and antioxidant activities, namely, syringaldehyde and syringic acid (Figure 7). The anti-inflammatory properties of these two low molecular weight organic compounds were proven by their inhibition of the mediator cyclooxygenase-2 (COX-2) enzyme in mouse macrophage (RAW 264.7). The crude ethanolic extract caused about 68% inhibition of the enzyme activity at a concentration of 50 μg/mL. The inhibitory effect of the purified syringaldehyde and syringic acid on COX-2 activity showed a dose-dependent effect, with an IC50 of 3.5 and 0.4 μg/mL, respectively, [19, 96].

Agaricoglycerides represent a new class of fungal secondary metabolites that constitute esters of chlorinated 4-hydroxy benzoic acid and glycerol and are produced in the culture of different edible mushrooms such as Grifola frondosa and Agaricus macrosporus. They also exhibited significant anti-inflammatory activity. The agaricoglycerides isolated from Grifola frondosa (AGF) showed potent anti-inflammatory activity since they were able to reduce the level of many mediators, such as IL-1β, NF-κB, ICAM-1, COX-2, and iNOS in animal models [97]. This study showed that an oral dose of 500 mg/kg/day of the ethanolic extract of an agaricoglyceride mixture showed strong anti-inflammatory activity in a Wister rat animal model.

4. Future Perspectives

This review has demonstrated the importance of mushrooms as potential biofactories for the production of natural anti-inflammatory metabolites of highly diversified chemical structure. The bioactivities of these compounds are exhibited through the downregulation of different types of inflammatory mediators. In addition to the high potential application of anti-inflammatory metabolites from mushrooms in forms of unpurified extract and extra pure compounds in medical applications, they can also be used in cosmeceutical products as safe and natural active ingredients without undesired side effects. However, the future medical application of anti-inflammatory compounds isolated from mushrooms faces five main challenges. Firstly, most of the studied mushrooms are not cultivable in greenhouses, and thus their availability is both seasonal and highly affected by changes in the weather. Secondly, the contents of the bioactive ingredients vary widely between samples, dependent on the collection time and procedure, the season, and the environment. Thirdly, mushroom cultivation in greenhouses is an open system and is not run according to the current Good Manufacturing Practice (cGMP) requirements for the production of bioactive medicinal compounds. Therefore, more research should be done on the development of mushroom cultivation processes in submerged cultures under fully sterile conditions so as to produce bioactive metabolites for pharmaceutical applications. Fourthly, in most of the studies conducted so far, the anti-inflammatory activities of mushrooms were demonstrated using crude mushroom extracts or solvent extracts of different metabolites in a mixed form. It is necessary, therefore, to isolate and identify the active metabolites for a better understanding of the anti-inflammatory properties of each particular compound and the possible side effects, if any. This step is necessary to upgrade the crude extract from a nutraceutical to pharmaceutical market after proper product formulation and clinical trials to determine the proper dose and claims. Fifthly, there is a lack of validated standard testing protocols to guarantee the quality and the efficacy of mushroom products for pharmaceutical applications. Overall, therefore, more research is required to overcome the challenges mentioned above before macrofungi can be fully accepted as one of the major biofactories for the production of anti-inflammatory medicine.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Acknowledgments

The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the Research Group Project no. RGP-VPP-284. The authors are also thankful for Research Management Centre, Universiti Teknologi Malaysia.

References

  1. W. E. Smalley, W. A. Ray, J. R. Daugherty, and M. R. Griffin, “Nonsteroidal anti-inflammatory drugs and the incidence of hospitalizations for peptic ulcer disease in elderly persons,” American Journal of Epidemiology, vol. 141, no. 6, pp. 539–545, 1995. View at: Google Scholar
  2. M. Sinha, L. Gautam, P. K. Shukla, P. Kaur, S. Sharma, and T. P. Singh, “Current perspectives in NSAID-induced gastropathy,” Mediators of Inflammation, vol. 2013, Article ID 258209, 11 pages, 2013. View at: Publisher Site | Google Scholar
  3. C. E. Dugowson and P. Gnanashanmugam, “Nonsteroidal anti-inflammatory drugs,” Physical Medicine and Rehabilitation Clinics of North America, vol. 17, no. 2, pp. 347–354, 2006. View at: Publisher Site | Google Scholar
  4. E. L. Fosbøl, G. H. Gislason, S. Jacobsen et al., “Risk of myocardial infarction and death associated with the use of nonsteroidal anti-inflammatory drugs (NSAIDs) among healthy individuals: a nationwide cohort study,” Clinical Pharmacology & Therapeutics, vol. 85, no. 2, pp. 190–197, 2009. View at: Publisher Site | Google Scholar
  5. I. L. Meek, M. A. F. J. van de Laar, and H. E. Vonkeman, “Non-steroidal anti-inflammatory drugs: an overview of cardiovascular risks,” Pharmaceuticals, vol. 3, no. 7, pp. 2146–2162, 2010. View at: Publisher Site | Google Scholar
  6. G. Yuan, M. L. Wahlqvist, G. He, M. Yang, and D. Li, “Natural products and anti-inflammatory activity,” Asia Pacific Journal of Clinical Nutrition, vol. 15, no. 2, pp. 143–152, 2006. View at: Google Scholar
  7. R. de Cássia da Silveira E Sá, L. N. Andrade, and D. P. de Sousa, “A review on anti-inflammatory activity of monoterpenes,” Molecules, vol. 18, no. 1, pp. 1227–1254, 2013. View at: Publisher Site | Google Scholar
  8. Y. Bellik, L. Boukraâ, H. A. Alzahrani et al., “Molecular mechanism underlying anti-inflammatory and anti-Allergic activities of phytochemicals: an update,” Molecules, vol. 18, no. 1, pp. 322–353, 2013. View at: Publisher Site | Google Scholar
  9. A. Gomes, E. Fernandes, J. L. F. C. Lima, L. Mira, and M. L. Corvo, “Molecular mechanisms of anti-inflammatory activity mediated by flavonoids,” Current Medicinal Chemistry, vol. 15, no. 16, pp. 1586–1605, 2008. View at: Publisher Site | Google Scholar
  10. S. S. Mendes, R. R. Bomfim, H. C. R. Jesus et al., “Evaluation of the analgesic and anti-inflammatory effects of the essential oil of Lippia gracilis leaves,” Journal of Ethnopharmacology, vol. 129, no. 3, pp. 391–397, 2010. View at: Publisher Site | Google Scholar
  11. M. G. Miguel, “Antioxidant and anti-inflammatory activities of essential oils: a short review,” Molecules, vol. 15, no. 12, pp. 9252–9287, 2010. View at: Publisher Site | Google Scholar
  12. O. V. Oyetayo, “Medicinal uses of mushrooms in Nigeria: towards full and sustainable exploitation,” African Journal of Traditional, Complementary and Alternative Medicines, vol. 8, no. 3, pp. 267–274, 2011. View at: Google Scholar
  13. D. D. Tibuhwa, “Folk taxonomy and use of mushrooms in communities around Ngorongoro and Serengeti National Park, Tanzania,” Journal of Ethnobiology and Ethnomedicine, vol. 8, article 36, 2012. View at: Publisher Site | Google Scholar
  14. H. El Enshasy, E. A. Elsayed, R. Aziz, and M. A. Wadaan, “Mushrooms and truffles: historical biofactories for complementary medicine in Africa and in the middle East,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 620451, 10 pages, 2013. View at: Publisher Site | Google Scholar
  15. J. M. Trappe, A. W. Claridge, D. L. Claridge, and L. Liddle, “Desert truffles of the Australian outback: ecology, ethnomycology, and taxonomy,” Economic Botany, vol. 62, no. 3, pp. 497–506, 2008. View at: Publisher Site | Google Scholar
  16. S. Badalyan, “Medicinal aspects of edible ectomycorrhizal mushrooms,” in Edible Ectomycorrhizal Mushrooms, pp. 317–334, Springer, Berlin, Germany, 2012. View at: Google Scholar
  17. A. Colak, F. Özlem, and E. Sesli, “Nutritional composition of some wild edible mushrooms,” Turkish Journal of Biochemistry, vol. 34, no. 1, pp. 25–31, 2009. View at: Google Scholar
  18. P. Kalač, “A review of chemical composition and nutritional value of wild-growing and cultivated mushrooms,” Journal of the Science of Food and Agriculture, vol. 93, no. 2, pp. 209–218, 2013. View at: Publisher Site | Google Scholar
  19. S. Wang and M. F. Marcone, “The biochemistry and biological properties of the world's most expensive underground edible mushroom: truffles,” Food Research International, vol. 44, no. 9, pp. 2567–2581, 2011. View at: Publisher Site | Google Scholar
  20. A. Villares, A. García-Lafuente, E. Guillamón, and Á. Ramos, “Identification and quantification of ergosterol and phenolic compounds occurring in Tuber spp. truffles,” Journal of Food Composition and Analysis, vol. 26, no. 1-2, pp. 177–182, 2012. View at: Publisher Site | Google Scholar
  21. J.-Z. He, Q.-M. Ru, D.-D. Dong, and P.-L. Sun, “Chemical characteristics and antioxidant properties of crude water soluble polysaccharides from four common edible mushrooms,” Molecules, vol. 17, no. 4, pp. 4373–4387, 2012. View at: Publisher Site | Google Scholar
  22. H. El Enshasy, “Immunomodulators,” in The Mycota: Industrial Applications, vol. 10, pp. 165–194, Springer, Berlin, Germany, 2010. View at: Google Scholar
  23. D. Yamanaka, R. Tada, Y. Adachi et al., “Agaricus brasiliensis-derived β-glucans exert immunoenhancing effects via a dectin-1-dependent pathway,” International Immunopharmacology, vol. 14, no. 3, pp. 311–319, 2012. View at: Publisher Site | Google Scholar
  24. X.-Q. Han, X.-M. Wu, X.-Y. Chai et al., “Isolation, characterization and immunological activity of a polysaccharide from the fruit bodies of an edible mushroom, Sarcodon aspratus (Berk.) S. Ito,” Food Research International, vol. 44, no. 1, pp. 489–493, 2011. View at: Publisher Site | Google Scholar
  25. H. A. El Enshasy and R. Hatti-Kaul, “Mushroom immunomodulators: unique molecules with unlimited applications,” Trends in Biotechnology, vol. 31, no. 12, pp. 668–677, 2013. View at: Publisher Site | Google Scholar
  26. D. Sliva, J. Loganathan, J. Jiang et al., “Mushroom Ganoderma lucidum prevents colitis-associated carcinogenesis in mice,” PLoS ONE, vol. 7, no. 10, Article ID e47873, 2012. View at: Publisher Site | Google Scholar
  27. A. Zong, H. Cao, and F. Wang, “Anticancer polysaccharides from natural resources: a review of recent research,” Carbohydrate Polymers, vol. 90, no. 4, pp. 395–410, 2012. View at: Publisher Site | Google Scholar
  28. M.-F. Moradali, H. Mostafavi, S. Ghods, and G.-A. Hedjaroude, “Immunomodulating and anticancer agents in the realm of macromycetes fungi (macrofungi),” International Immunopharmacology, vol. 7, no. 6, pp. 701–724, 2007. View at: Publisher Site | Google Scholar
  29. Y.-J. Liu, J. Shen, Y.-M. Xia, J. Zhang, and H.-S. Park, “The polysaccharides from Ganoderma lucidum: are they always inhibitors on human hepatocarcinoma cells?” Carbohydrate Polymers, vol. 90, no. 3, pp. 1210–1215, 2012. View at: Publisher Site | Google Scholar
  30. H. El-Enshasy, A. Daba, M. El-Demellawy, A. Ibrahim, S. El Sayed, and I. El-Badry, “Bioprocess development for large scale production of anticancer exo-polysaccharide by Pleurotus ostreatus in submerged culture,” Journal of Applied Sciences, vol. 10, no. 21, pp. 2523–2529, 2010. View at: Publisher Site | Google Scholar
  31. S. Janakat, S. Al-Fakhiri, and A.-K. Sallal, “A promising peptide antibiotic from Terfezia claveryi aqueous extract against Staphylococcus aureusin vitro,” Phytotherapy Research, vol. 18, no. 10, pp. 810–813, 2004. View at: Publisher Site | Google Scholar
  32. S. M. Janakat, S. M. Al-Fakhiri, and A.-K. J. Sallal, “Evaluation of antibacterial activity of aqueous and methanolic extracts of the truffle Terfezia claveryi against Pseudomonas aeruginosa,” Saudi Medical Journal, vol. 26, no. 6, pp. 952–955, 2005. View at: Google Scholar
  33. G. Adotey, A. Quarcoo, J. C. Holliday, S. Fofie, and B. Saaka, “Effect of immunomodulating and antiviral agent of medicinal mushrooms (immune assist 24/7) on CD4+ T-Lymphocyte Counts of HIV-infected patients,” International Journal of Medicinal Mushrooms, vol. 13, no. 2, pp. 109–113, 2011. View at: Publisher Site | Google Scholar
  34. J.-Y. Yeh, L.-H. Hsieh, K.-T. Wu, and C.-F. Tsai, “Antioxidant properties and antioxidant compounds of various extracts from the edible basidiomycete Grifola frondosa (Maitake),” Molecules, vol. 16, no. 4, pp. 3197–3211, 2011. View at: Publisher Site | Google Scholar
  35. Z. Wang and D. Luo, “Antioxidant activities of different fractions of polysaccharide purified from Gynostemma pentaphyllum Makino,” Carbohydrate Polymers, vol. 68, no. 1, pp. 54–58, 2007. View at: Publisher Site | Google Scholar
  36. K. N. Yoon, N. Alam, K. R. Lee et al., “Antioxidant and antityrosinase activities of various extracts from the fruiting bodies of Lentinus lepideus,” Molecules, vol. 16, no. 3, pp. 2334–2347, 2011. View at: Publisher Site | Google Scholar
  37. H. Mu, A. Zhang, W. Zhang, G. Cui, S. Wang, and J. Duan, “Antioxidative properties of crude polysaccharides from Inonotus obliquus,” International Journal of Molecular Sciences, vol. 13, no. 7, pp. 9194–9206, 2012. View at: Publisher Site | Google Scholar
  38. S.-H. Hu, J.-C. Wang, J.-L. Lien, E.-T. Liaw, and M.-Y. Lee, “Antihyperglycemic effect of polysaccharide from fermented broth of Pleurotus citrinopileatus,” Applied Microbiology and Biotechnology, vol. 70, no. 1, pp. 107–113, 2006. View at: Publisher Site | Google Scholar
  39. A.-H. Kwon, Z. Qiu, M. Hashimoto, K. Yamamoto, and T. Kimura, “Effects of medicinal mushroom (Sparassis crispa) on wound healing in streptozotocin-induced diabetic rats,” The American Journal of Surgery, vol. 197, no. 4, pp. 503–509, 2009. View at: Publisher Site | Google Scholar
  40. H. C. Lo and S. P. Wasser, “Medicinal mushrooms for glycemic control in diabetes mellitus: History, current status, future perspectives, and unsolved problems (review),” International Journal of Medicinal Mushrooms, vol. 13, no. 5, pp. 401–426, 2011. View at: Publisher Site | Google Scholar
  41. E. Guillamón, A. García-Lafuente, M. Lozano et al., “Edible mushrooms: role in the prevention of cardiovascular diseases,” Fitoterapia, vol. 81, no. 7, pp. 715–723, 2010. View at: Publisher Site | Google Scholar
  42. C. Nathan, “Points of control in inflammation,” Nature, vol. 420, no. 6917, pp. 846–852, 2002. View at: Publisher Site | Google Scholar
  43. J. M. Cook-Mills and T. L. Deem, “Active participation of endothelial cells in inflammation,” Journal of Leukocyte Biology, vol. 77, no. 4, pp. 487–495, 2005. View at: Publisher Site | Google Scholar
  44. T. B. Levine and A. B. Levine, “Inflammation,” in Metabolic Syndrome and Cardiovascular Disease, pp. 192–227, Blackwell Publishing, Oxford, UK, 2012. View at: Google Scholar
  45. L. M. Coussens and Z. Werb, “Inflammation and cancer,” Nature, vol. 420, no. 6917, pp. 860–867, 2002. View at: Publisher Site | Google Scholar
  46. A. Mantovani, P. Allavena, A. Sica, and F. Balkwill, “Cancer-related inflammation,” Nature, vol. 454, no. 7203, pp. 436–444, 2008. View at: Publisher Site | Google Scholar
  47. S. P. Wasser and A. L. Weis, “Medicinal properties of substances occurring in higher Basidiomycetes mushrooms: current prespectives,” International Journal of Medicinal Mushrooms, vol. 1, pp. 31–62, 1999. View at: Publisher Site | Google Scholar
  48. S. T. Chang and P. G. Miles, Mushrooms: Cultivation, Nutritional Value, Medicinal Eeffect and Environmental Impact, CRC Press, Boca Raton, Fla, USA, 1st edition, 2004.
  49. S. Bernardshaw, E. Johnson, and G. Hetland, “An extract of the mushroom Agaricus blazei murill administered orally protects against systemic Streptococcus pneumoniae infection in mice,” Scandinavian Journal of Immunology, vol. 62, no. 4, pp. 393–398, 2005. View at: Publisher Site | Google Scholar
  50. J. L. Gonçalves, E. H. Roma, A. C. Gomes-Santos et al., “Pro-inflammatory effects of the mushroom Agaricus blazei and its consequences on atherosclerosis development,” European Journal of Nutrition, vol. 51, no. 8, pp. 927–937, 2012. View at: Publisher Site | Google Scholar
  51. H.-H. Song, H.-S. Chae, S.-R. Oh, H.-K. Lee, and Y.-W. Chin, “Anti-inflammatory and anti-allergic effect of Agaricus blazei extract in bone marrow-derived mast cells,” The American Journal of Chinese Medicine, vol. 40, no. 5, pp. 1073–1084, 2012. View at: Publisher Site | Google Scholar
  52. E. Johnson, D. T. Førland, L. Sætre, S. V. Bernardshaw, T. Lyberg, and G. Hetland, “Effect of an extract based on the medicinal mushroom agaricus blazei murill on release of cytokines, chemokines and leukocyte growth factors in human blood ex vivo and in vivo,” Scandinavian Journal of Immunology, vol. 69, no. 3, pp. 242–250, 2009. View at: Publisher Site | Google Scholar
  53. D. T. Førland, E. Johnson, L. Sætre, T. Lyberg, I. Lygren, and G. Hetland, “Effect of an extract based on the medicinal mushroom Agaricus blazei Murill on expression of cytokines and calprotectin in patients with ulcerative colitis and Crohn’s disease,” Scandinavian Journal of Immunology, vol. 73, no. 1, pp. 66–75, 2011. View at: Publisher Site | Google Scholar
  54. L. K. Ellertsen and G. Hetland, “An extract of the medicinal mushroom Agaricus blazei Murill can protect against allergy,” Clinical and Molecular Allergy, vol. 7, article 6, 2009. View at: Publisher Site | Google Scholar
  55. K. Wisitrassameewong, S. C. Karunarathna, N. Thongklang et al., “Agaricus subrufescens: a review,” Saudi Journal of Biological Sciences, vol. 19, no. 2, pp. 131–146, 2012. View at: Publisher Site | Google Scholar
  56. S. Bernardshaw, G. Hetland, L. K. Ellertsen, A. M. A. Tryggestad, and E. Johnson, “An extract of the medicinal mushroom Agaricus blazei Murill differentially stimulates production of pro-inflammatory cytokines in human monocytes and human vein endothelial cells in vitro,” Inflammation, vol. 29, no. 4–6, pp. 147–153, 2005. View at: Publisher Site | Google Scholar
  57. I. Lavi, L. Nimri, D. Levinson, I. Peri, Y. Hadar, and B. Schwartz, “Glucans from the edible mushroom Pleurotus pulmonarius inhibit colitis-associated colon carcinogenesis in mice,” Journal of Gastroenterology, vol. 47, no. 5, pp. 504–518, 2012. View at: Publisher Site | Google Scholar
  58. I. Lavi, D. Levinson, I. Peri, L. Nimri, Y. Hadar, and B. Schwartz, “Orally administered glucans from the edible mushroom Pleurotus pulmonarius reduce acute inflammation in dextran sulfate sodium-induced experimental colitis,” British Journal of Nutrition, vol. 103, no. 3, pp. 393–402, 2010. View at: Publisher Site | Google Scholar
  59. B. Schwartz, Y. Hadar, and D. Silva, “The use of edible mushroom water soluble polysaccharides in the treatment and prevention of chronic diseases: a mechanistic approach,” in Antitumor Potential and other Emerging Medicinal Properties of Natural Compounds, pp. 263–283, Springer, Dordrecht, The Netherlands, 2013. View at: Google Scholar
  60. F. R. Smirdele, L. M. Olsen, E. R. Carbonero et al., “Anti-inflammatory and analgesic properties in a rodent model (1→3), (1→6)-linked-glucan isolated from Pleurotus pulmonarius,” European Journal of Pharmacology, vol. 597, pp. 8–91, 2008. View at: Google Scholar
  61. P. Bobek, V. Nosalova, and S. Cerna, “Effect of pleuran (beta-glucan from Pleurotus ostreatus) in diet or drinking fluid on colitis in rats,” Nahrung, vol. 45, pp. 360–363, 2001. View at: Google Scholar
  62. A. Jedinak, S. Dudhgaonkar, Q.-L. Wu, J. Simon, and D. Sliva, “Anti-inflammatory activity of edible oyster mushroom is mediated through the inhibition of NF-κB and AP-1 signaling,” Nutrition Journal, vol. 10, no. 1, article 52, 2011. View at: Publisher Site | Google Scholar
  63. L. S. Queiroz, M. S. Nascimento, A. K. M. Cruz et al., “Glucans from the Caripia montagnei mushroom present anti-inflammatory activity,” International Immunopharmacology, vol. 10, no. 1, pp. 34–42, 2010. View at: Publisher Site | Google Scholar
  64. A. C. Ruthes, E. R. Carbonero, M. M. Córdova et al., “Lactarius rufus (13),(16)-β-d-glucans: structure, antinociceptive and anti-inflammatory effects,” Carbohydrate Polymers, vol. 94, no. 1, pp. 129–136, 2013. View at: Publisher Site | Google Scholar
  65. A. C. Ruthes, Y. D. Rattmann, S. M. Malquevicz-Paiva et al., “Agaricus bisporus fucogalactan: structural characterization and pharmacological approaches,” Carbohydrate Polymers, vol. 92, no. 1, pp. 184–191, 2013. View at: Publisher Site | Google Scholar
  66. E. R. Carbonero, A. H. P. Gracher, D. L. Komura et al., “Lentinus edodes heterogalactan: antinociceptive and anti-inflammatory effects,” Food Chemistry, vol. 111, no. 3, pp. 531–537, 2008. View at: Publisher Site | Google Scholar
  67. N. Fangkrathok, J. Junlatat, and B. Sripanidkulchai, “In vivo and in vitro anti-inflammatory activity of Lentinus polychrous extract,” Journal of Ethnopharmacology, vol. 147, no. 3, pp. 631–637, 2013. View at: Publisher Site | Google Scholar
  68. Y.-Y. Lu, Z.-H. Ao, Z.-M. Lu et al., “Analgesic and anti-inflammatory effects of the dry matter of culture broth of Termitomyces albuminosus and its extracts,” Journal of Ethnopharmacology, vol. 120, no. 3, pp. 432–436, 2008. View at: Publisher Site | Google Scholar
  69. S.-H. Kim, Y.-S. Song, S.-K. Kim, B.-C. Kim, C.-J. Lim, and E.-H. Park, “Anti-inflammatory and related pharmacological activities of the n-BuOH subfraction of mushroom Phellinus linteus,” Journal of Ethnopharmacology, vol. 93, no. 1, pp. 141–146, 2004. View at: Publisher Site | Google Scholar
  70. B.-C. Kim, J.-W. Choi, H.-Y. Hong et al., “Heme oxygenase-1 mediates the anti-inflammatory effect of mushroom Phellinus linteus in LPS-stimulated RAW264.7 macrophages,” Journal of Ethnopharmacology, vol. 106, no. 3, pp. 364–371, 2006. View at: Publisher Site | Google Scholar
  71. H. Li, X. Lu, S. Zhang, M. Lu, and H. Liu, “Anti-inflammatory activity of polysaccharide from Pholiota nameko,” Biochemistry, vol. 73, no. 6, pp. 669–675, 2008. View at: Publisher Site | Google Scholar
  72. D.-M. Wu, W.-Q. Duan, Y. Liu, and Y. Cen, “Anti-inflammatory effect of the polysaccharides of Golden needle mushroom in burned rats,” International Journal of Biological Macromolecules, vol. 46, no. 1, pp. 100–103, 2010. View at: Publisher Site | Google Scholar
  73. R. O. Diniz, L. K. Garla, J. M. Schneedorf, and J. C. T. Carvalho, “Study of anti-inflammatory activity of Tibetan mushroom, a symbiotic culture of bacteria and fungi encapsulated into a polysaccharide matrix,” Pharmacological Research, vol. 47, no. 1, pp. 49–52, 2003. View at: Publisher Site | Google Scholar
  74. I. Tsvetkova, H. Naydenski, A. Petrova et al., “Antibacterial activity of some Bulgarian higher Basidiomycetes mushrooms,” International Journal of Medicinal Mushrooms, vol. 8, no. 1, pp. 63–66, 2006. View at: Publisher Site | Google Scholar
  75. H. Fujimoto, Y. Nakayama, and M. Yamazaki, “Identification of immunosuppressive components of a mushroom, Lactarius flavidulus,” Chemical & Pharmaceutical Bulletin, vol. 41, no. 4, pp. 654–658, 1993. View at: Publisher Site | Google Scholar
  76. J. Han, Y. Chen, L. Bao et al., “Anti-inflammatory and cytotoxic cyathane diterpenoids from the medicinal fungus Cyathus africanus,” Fitoterapia, vol. 84, no. 1, pp. 22–31, 2013. View at: Publisher Site | Google Scholar
  77. Z. Xu, S. Yan, K. Bi et al., “Isolation and identification of a new anti-inflammatory cyathane diterpenoid from the medicinal fungus Cyathus hookeriBerk,” Fitoterapia, vol. 86, no. 1, pp. 159–162, 2013. View at: Publisher Site | Google Scholar
  78. S. Dudhgaonkar, A. Thyagarajan, and D. Sliva, “Suppression of the inflammatory response by triterpenes isolated from the mushroom Ganoderma lucidum,” International Immunopharmacology, vol. 9, no. 11, pp. 1272–1280, 2009. View at: Publisher Site | Google Scholar
  79. K. Iwatsuki, T. Akihisa, H. Tokuda et al., “Lucidenic acids P and Q, methyl lucidenate P, and other triterpenoids from the fungus Ganoderma lucidum and their inhibitory effects on Epstein-Barr virus activation,” Journal of Natural Products, vol. 66, no. 12, pp. 1582–1585, 2003. View at: Publisher Site | Google Scholar
  80. T. Akihisa, M. Tagata, M. Ukiya, H. Tokuda, T. Suzuki, and Y. Kimura, “Oxygenated lanostane-type triterpenoids from the fungus Ganoderma lucidum,” Journal of Natural Products, vol. 68, no. 4, pp. 559–563, 2005. View at: Publisher Site | Google Scholar
  81. T. Akihisa, Y. Nakamura, M. Tagata et al., “Anti-inflammatory and anti-tumor-promoting effects of triterpene acids and sterols from the fungus Ganoderma lucidum,” Chemistry and Biodiversity, vol. 4, no. 2, pp. 224–231, 2007. View at: Publisher Site | Google Scholar
  82. Q. Van, B. N. Nayak, M. Reimer, P. J. H. Jones, R. G. Fulcher, and C. B. Rempel, “Anti-inflammatory effect of Inonotus obliquus, Polygala senega L., and Viburnum trilobum in a cell screening assay,” Journal of Ethnopharmacology, vol. 125, no. 3, pp. 487–493, 2009. View at: Publisher Site | Google Scholar
  83. Y.-M. Park, J.-H. Won, Y.-H. Kim, J.-W. Choi, H.-J. Park, and K.-T. Lee, “In vivo and in vitro anti-inflammatory and anti-nociceptive effects of the methanol extract of Inonotus obliquus,” Journal of Ethnopharmacology, vol. 101, no. 1–3, pp. 120–128, 2005. View at: Publisher Site | Google Scholar
  84. L. Ma, H. Chen, P. Dong, and X. Lu, “Anti-inflammatory and anticancer activities of extracts and compounds from the mushroom Inonotus obliquus,” Food Chemistry, vol. 139, no. 1–4, pp. 503–508, 2013. View at: Publisher Site | Google Scholar
  85. J. Wang, Y.-M. Liu, W. Cao, K.-W. Yao, Z.-Q. Liu, and J.-Y. Guo, “Anti-inflammation and antioxidant effect of cordymin, a peptide purified from the medicinal mushroom Cordyceps sinensis, in middle cerebral artery occlusion-induced focal cerebral ischemia in rats,” Metabolic Brain Disease, vol. 27, no. 2, pp. 159–165, 2012. View at: Publisher Site | Google Scholar
  86. G.-M. Qian, G.-F. Pan, and J.-Y. Guo, “Anti-inflammatory and antinociceptive effects of cordymin, a peptide purified from the medicinal mushroom Cordyceps sinensis,” Natural Product Research, vol. 26, no. 24, pp. 2358–2362, 2012. View at: Publisher Site | Google Scholar
  87. J. H. Wong, T. B. Ng, H. Wang et al., “Cordymin, an antifungal peptide from the medicinal fungus Cordyceps militaris,” Phytomedicine, vol. 18, no. 5, pp. 387–392, 2011. View at: Publisher Site | Google Scholar
  88. Y. Zhang, G. L. Mills, and M. G. Nair, “Cyclooxygenase inhibitory and antioxidant compounds from the fruiting body of an edible mushroom, Agrocybe aegerita,” Phytomedicine, vol. 10, no. 5, pp. 386–390, 2003. View at: Publisher Site | Google Scholar
  89. B. Dugler, A. Gonuz, and F. Gucin, “Antimicrobial activity of the macrofungus Cantharellus cibarius,” Pakistan Journal of Biological Sciences, vol. 7, pp. 1535–1539, 2004. View at: Google Scholar
  90. A. M. Witkowska, M. E. Zujko, and I. Mirończuk-Chodakowska, “Comparative study of wild edible mushrooms as sources of antioxidants,” International Journal of Medicinal Mushrooms, vol. 13, no. 4, pp. 335–341, 2011. View at: Publisher Site | Google Scholar
  91. C. Moro, I. Palacios, M. Lozano et al., “Anti-inflammatory activity of methanolic extracts from edible mushrooms in LPS activated RAW 264.7 macrophages,” Food Chemistry, vol. 130, no. 2, pp. 350–355, 2012. View at: Publisher Site | Google Scholar
  92. D. N. Quang, L. Harinantenaina, T. Nishizawa et al., “Inhibitory activity of nitric oxide production in RAW 264.7 cells of daldinals A-C from the fungus Daldinia childiae and other metabolites isolated from inedible mushrooms,” Journal of Natural Medicines, vol. 60, no. 4, pp. 303–307, 2006. View at: Publisher Site | Google Scholar
  93. M. Nukata, T. Hashimoto, I. Yamamoto, N. Iwasaki, M. Tanaka, and Y. Asakawa, “Neogrifolin derivatives possessing anti-oxidative activity from the mushroom Albatrellus ovinus,” Phytochemistry, vol. 59, no. 7, pp. 731–737, 2002. View at: Publisher Site | Google Scholar
  94. D. N. Quang, T. Hashimoto, Y. Arakawa et al., “Grifolin derivatives from Albatrellus caeruleoporus, new inhibitors of nitric oxide production in RAW 264.7 cells,” Bioorganic and Medicinal Chemistry, vol. 14, no. 1, pp. 164–168, 2006. View at: Publisher Site | Google Scholar
  95. M. Ye, J. K. Liu, Z. X. Lu et al., “Grifolin, a potential antitumor natural product from the mushroom Albatrellus confluens, inhibits tumor cell growth by inducing apoptosis in vitro,” FEBS Letters, vol. 579, no. 16, pp. 3437–3443, 2005. View at: Publisher Site | Google Scholar
  96. R. Stanikunaite, S. I. Khan, J. M. Trappe, and S. A. Ross, “Cyclooxygenase-2 inhibitory and antioxidant compounds from the truffle Elaphomyces granulatus,” Phytotherapy Research, vol. 23, no. 4, pp. 575–578, 2009. View at: Publisher Site | Google Scholar
  97. C. Han and B. Cui, “Pharmacological and pharmacokinetic studies with agaricoglycerides, extracted from Grifola frondosa, in animal models of pain and inflammation,” Inflammation, vol. 35, no. 4, pp. 1269–1275, 2012. View at: Publisher Site | Google Scholar
  98. P. H. K. Ngai, Z. Zhao, and T. B. Ng, “Agrocybin, an antifungal peptide from the edible mushroom Agrocybe cylindracea,” Peptides, vol. 26, no. 2, pp. 191–196, 2005. View at: Publisher Site | Google Scholar
  99. D. Michelot and L. M. Melendez-Howell, “Amanita muscaria: chemistry, biology, toxicology, and ethnomycology,” Mycological Research, vol. 107, no. 2, pp. 131–146, 2003. View at: Publisher Site | Google Scholar
  100. S.-Y. Won and E.-H. Park, “Anti-inflammatory and related pharmacological activities of cultured mycelia and fruiting bodies of Cordyceps militaris,” Journal of Ethnopharmacology, vol. 96, no. 3, pp. 555–561, 2005. View at: Publisher Site | Google Scholar
  101. K.-M. Kim, Y.-G. Kwon, H.-T. Chung et al., “Methanol extract of Cordyceps pruinosa inhibits in vitro and in vivo inflammatory mediators by suppressing NF-κB activation,” Toxicology and Applied Pharmacology, vol. 190, no. 1, pp. 1–8, 2003. View at: Publisher Site | Google Scholar
  102. J.-J. Cheng, C.-Y. Lin, H.-S. Lur, H.-P. Chen, and M.-K. Lu, “Properties and biological functions of polysaccharides and ethanolic extracts isolated from medicinal fungus, Fomitopsis pinicola,” Process Biochemistry, vol. 43, no. 8, pp. 829–834, 2008. View at: Publisher Site | Google Scholar
  103. Y. Zhang, G. L. Mills, and M. G. Nair, “Cyclooxygenase inhibitory and antioxidant compounds from the mycelia of the edible mushroom Grifola frondosa,” Journal of Agricultural and Food Chemistry, vol. 50, no. 26, pp. 7581–7585, 2002. View at: Publisher Site | Google Scholar
  104. C. M. P. Guerra Dore, T. C. G. Azevedo, M. C. R. de Souza et al., “Antiinflammatory, antioxidant and cytotoxic actions of β-glucan-rich extract from Geastrum saccatum mushroom,” International Immunopharmacology, vol. 7, no. 9, pp. 1160–1169, 2007. View at: Publisher Site | Google Scholar
  105. T. Debnath, M. A. Hasnat, M. Pervin et al., “Chaga mushroom (Inonotus obliquus) grown on germinated brown rice suppresses inflammation associated with colitis in mice,” Food Science and Biotechnology, vol. 21, no. 5, pp. 1235–1241, 2012. View at: Publisher Site | Google Scholar
  106. Y. Ukawa, H. Ito, and M. Hisamatsu, “Antitumor effects of (13)-β-D-glucan and (16)-β-D-glucan purified from newly cultivated mushroom, Hatakeshimeji (Lyophyllum decastes Sing),” Journal of Bioscience and Bioengineering, vol. 90, no. 1, pp. 98–104, 2000. View at: Publisher Site | Google Scholar
  107. B.-C. Kim, W.-K. Jeon, H.-Y. Hong et al., “The anti-inflammatory activity of Phellinus linteus (Berk. & M.A. Curt.) is mediated through the PKCδ/Nrf2/ARE signaling to up-regulation of heme oxygenase-1,” Journal of Ethnopharmacology, vol. 113, no. 2, pp. 240–247, 2007. View at: Publisher Site | Google Scholar
  108. M.-K. Lu, J.-J. Cheng, C.-Y. Lin, and C.-C. Chang, “Purification, structural elucidation, and anti-inflammatory effect of a water-soluble 1,6-branched 1,3-α-d-galactan from cultured mycelia of Poria cocos,” Food Chemistry, vol. 118, no. 2, pp. 349–356, 2010. View at: Publisher Site | Google Scholar

Copyright © 2014 Elsayed A. Elsayed et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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