Scropolioside B Inhibits IL-1β and Cytokines Expression through NF-κB and Inflammasome NLRP3 Pathways

Figure 1

Different chemical structures of scropolioside B and catalpol. (a) The effects of scropolioside B and catalpol on LPS-induced expression of IL-1β and TNF-α in THP-1 cells. THP-1 cells were pretreated with 50 μmol/L catalpol or scropolioside B for 1 h and then stimulated with LPS (1 μg/mL) for another 24 h. ((b)-(c)) The effects of scropolioside B and catalpol on PA-induced expression of IL-1β and TNF-α in THP-1 cells. THP-1 cells were pretreated with 50 μmol/L catalpol or scropolioside B for 1 h and then stimulated with PA (50 μg/mL) for another 24 h. ((d)-(e)) The expression of IL-1β and TNF-α mRNA was measured by RT-PCR. The data represent the mean values of over three experiments ± SD. compared to vehicle control, compared to LPS or PA alone. compared to vehicle control, compared to LPS or PA alone.

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