Research Article
Scropolioside B Inhibits IL-1β and Cytokines Expression through NF-κB and Inflammasome NLRP3 Pathways
Table 1
Primer sequences of the genes tested in this study.
| | Gene | Direction | Primer sequences |
| | IL-1β | Forward | 5-AAACAGATGAAGTGCTCCTTCCAGG | | Reverse | 5-TGGAGAACACCACTTGTTGCTCCA |
| | TNF-α | Forward | 5-CAGAGGGAAGAGTTCCCCAG | | Reverse | 5′-CCTTGGTCTGGTAGGAGACG |
| | IL-32β | Forward | 5-GAGTTTCTGCTGCTCTCTGTCA | | Reverse | 5-ATTTTGAGGATTGGGGTTCAG |
| | IL-32γ | Forward | 5-GAGTTTCTGCTGCTCTCTGTCA | | Reverse | 5-ATTTTGAGGATTGGGGTTCAG |
| | NLRP3 | Forward | 5-CTACACACGACTGCGTCTCATCAA | | Reverse | 5-GGGTCAAACAGCAACTCCATCTTA |
| | CLS1 | Forward | 5-GAGTATGCCACAGTATGAAAACCCA | | Reverse | 5-CGAGCAATAAATCCATCCAACAA |
| | GAPDH | Forward | 5-AGAAGGCTGGGGCTCATTTG | | Reverse | 5-AGGGGCCATCCACAGTCTTC |
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