Metalloprotease Dependent Release of Placenta Derived Fractalkine
Figure 3
Analysis of fractalkine expression and release in the trophoblast cell line BeWo and primary term trophoblasts. Incubation with forskolin (20 μM) increased fractalkine mRNA expression in BeWo cells in a time dependent manner and showed a 22.1-fold (±1.6) upregulation compared to control (DMSO, 0.2%) after 48 h (a). Forskolin treatment of BeWo cells induced a 9.8-fold increase in secretion of soluble fractalkine compared to control after 48 h (b). In primary term trophoblasts secretion of soluble fractalkine increased between 24 h and 48 h incubation by 39.5% (c). Data in (a) and (b) are presented as mean ± SEM from three independent experiments performed in triplicate. Data in (c) are presented as mean ± SEM from four different trophoblast preparations. , , .
