Research Article
Effects of Central Injection of Anti-LPS Antibody and Blockade of TLR4 on GnRH/LH Secretion during Immunological Stress in Anestrous Ewes
Table 1
Specific primers used in real-time PCR for determining the expression of housekeeping genes and genes of interests.
| | Gene bank acc. number | Gene | Amplicon size [bp] | Forward/reverse | Sequence 5′→ 3′ |
| | NM_001034034 | GAPDH
glyceraldehyde-3-phosphate dehydrogenase | 134 | Forward | AGAAGGCTGGGGCTCACT | | Reverse | GGCATTGCTGACAATCTTGA |
| | U39357 | ACTB
beta actin | 168 | Forward | CTTCCTTCCTGGGCATGG | | Reverse | GGGCAGTGATCTCTTTCTGC |
| | NM_001076910 | PPIC
cyclophilin C | 131 | Forward | ACGGCCAAGGTCTTCTTTG | | Reverse | TATCCTTTCTCTCCCGTTGC |
| | U02517 | GnRH
gonadotropin-releasing hormone | 123 | Forward | GCCCTGGAGGAAAGAGAAAT | | Reverse | GAGGAGAATGGGACTGGTGA |
| | X52488 | LH-β
luteinizing hormone beta-subunit | 184 | Forward | AGATGCTCCAGGGACTGCT | | Reverse | TGCTTCATGCTGAGGCAGTA |
| | NM_001009397 | GnRH-R
gonadotropin-releasing hormone receptor | 150 | Forward | TCTTTGCTGGACCACAGTTAT | | Reverse | GGCAGCTGAAGGTGAAAAAG |
| | AY957615 | TLR4
Toll-like receptor 4 | 117 | Forward | GGTTCCCAGAACTGCAAGTG | | Reverse | GGATAGGGTTTCCCGTCAGT |
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