ChAT content within RAW264.7 cells, AChE concentration in the supernatant, and their mRNA expression level at the indicated time points of LPS exposure. (a) ChAT protein content in RAW264.7 cells lysates was detected by ELISA and the protein concentration of the lysates was determined by BAC method. The data were presented as pg ChAT per g protein. (b) AChE activity in supernatants of LPS treated RAW264.7 cells at the indicated time points, which was determined by the method of Ellman. (c) ChAT mRNA and (d) AChE mRNA expression levels in LPS treated RAW264.7 cells, which were determined by real-time PCR. Values are expressed as the ratio of ChAT or AChE to -actin. The data are representative of three individual experiments and presented as mean SD (), *, #, and & when compared with PBS, LPS 4 h, and LPS 8 h groups, respectively.
