ChAT mRNA expression level and ACh secretion level in RAW264.7 cells transfected with empty vector (EV) or ChAT expression vector (ChAT). (a) RAW264.7 cells transfected by ChAT expression vector under inverted phase contrast microscopy (100), which showed a good state. (b) GFP expression was more than 80% RAW264.7 cells infected by ChAT expression vector, under fluorescence microscopy (100). (c) ChAT mRNA expression level was upregulated largely in RAW264.7 cells transfected by ChAT expression vector. (d) The amount of ACh in the supernatant and lysate of ChAT overexpressed RAW264.7 cells; the determination of ACh was performed by ELISA. Data are representative of three independent experiments and expressed as mean SD (), * when compared with EV group.
