An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4⁺ T Cells

Figure 2

Luminex analysis for IL-1β, IL-4, IL-6, IL-10, IL-17A, IFN-γ, and TNF production by anti-CD3 plus anti-CD28 stimulated CD4⁺ peripheral blood T cells (; for TNF analysis, ) cultured for 3 days in the presence of two concentrations of CM derived from lamina propria mononuclear cells (LPMC) from inflamed CC mucosa or noninflamed controls. Cytokine amounts are expressed as cytokine amounts released by peripheral T cells incubated with CM minus cytokine amounts in CM alone, in pg/mL. For each blood donor, cytokine amounts released by peripheral T cells incubated with CM from CC patients were compared with the amounts released by peripheral T cells incubated with CM from noninflamed controls. Each symbol represents data from one donor and the data points from CC and noninflamed controls for each donor are connected with a line.