Purine-Metabolizing Ectoenzymes Control IL-8 Production in Human Colon HT-29 Cells
NTPDase2 is expressed by HT-29 cells. (a) HT-29 cells were incubated with ATP for 90 min and their supernatants were then subjected to RP-HPLC to quantify the various ATP degradation products (). (b) The expression of various human NTPDase genes was analyzed by semiquantitative RT-PCR where R, P, and N represent the reaction with HT-29 cells, the positive controls, and the negative controls, respectively, and (c) by quantitative RT-PCR (). (d) The expression of NTPDase proteins in HT-29 cells was analyzed by immunoblotting using specific antibodies to human NTPDase1, -2, -3, and -8, N1, N2, N3, and N8, respectively. A representative experiment of is shown.