Purine-Metabolizing Ectoenzymes Control IL-8 Production in Human Colon HT-29 Cells

Table 2

Adenine nucleotide hydrolysis at the surface of HT-29 cells and protein extracts.

Activities	Intact cells (𝑛=4); [nmoles Pi·min^−1·well^−1]	Cell lysates (𝑛=3); [nmoles Pi·min^−1·mg prot^−1]
ATPase	7.5 ± 0.3	115 ± 4
ADPase	1.5 ± 0.01	38 ± 1
AMPase	6.5 ± 0.3	67 ± 3

The hydrolysis of ATP, ADP, and AMP as substrate was performed and the liberated P_i was determined by the malachite green assay as detailed in Section 2.