Mediators of Inflammation / 2014 / Article / Tab 2

Research Article

Purine-Metabolizing Ectoenzymes Control IL-8 Production in Human Colon HT-29 Cells

Table 2

Adenine nucleotide hydrolysis at the surface of HT-29 cells and protein extracts.

ActivitiesIntact cells ( )
nmoles Pi·min−1·well−1
Cell lysates ( )
nmoles Pi·min−1·mg prot−1

ATPase7.5 ± 0.3115 ± 4
ADPase1.5 ± 0.0138 ± 1
AMPase6.5 ± 0.367 ± 3

The hydrolysis of ATP, ADP, and AMP as substrate was performed and the liberated Pi was determined by the malachite green assay as detailed in Section 2.