The Acute Phase of Trypanosoma cruzi Infection Is Attenuated in 5-Lipoxygenase-Deficient Mice

Figure 1

Lipid mediator production, parasitemia, tissue parasitism, and survival rate of WT and 5-LO^−/− mice infected with T. cruzi: ((a) and (b)) LTB₄ and PGE₂: peritoneal cells were collected from control, infected WT, and infected 5-LO^−/− mice (𝑛=10/group) and stimulated with calcium ionophore. ^∗𝑃<0.01 versus uninfected group; ^#𝑃<0.01 versus WT infected mice. (c) Parasitemia (𝑛=10 mice/group). ^#𝑃<0.001 versus infected WT mice. (d) Parasite nests in heart tissue on postinoculation day 16. ^∗𝑃<0.001 versus infected WT mice. (e) Survival: WT mice (squares) and 5-LO^−/− mice (triangles), 𝑛=10 animals/group. Wilcoxon signed-rank test (level of significance, 𝑃<0.001). Data are representative of three independent experiments.

(a)

(b)

(c)

(d)

(e)