Antagonizing Arachidonic Acid-Derived Eicosanoids Reduces Inflammatory Th17 and Th1 Cell-Mediated Inflammation and Colitis Severity
Table 2
Colonic mucosal eicosanoid and cannabinoid profiles in Fads1 TNBS-treated mice1.
Eicosanoid (ng/mg protein)
Wt
Het
Null
PGE1
1.91 ± 1.09*
1.87 ± 0.50*
16.36 ± 6.88‡
PGE2
15.70 ± 4.92*
14.19 ± 1.66*
1.92 ± 0.77‡
PGE3
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
PGD2
43.12 ± 4.51*
39.14 ± 2.51*
1.38 ± 0.06‡
5.94 ± 0.98*
4.15 ± 0.57*
0.11 ± 0.03‡
6-keto-
3.78 ± 0.98*
3.00 ± 0.74*
0.41 ± 0.11‡
TXB2
1.08 ± 0.23*
1.26 ± 0.21*
0.17 ± 0.02‡
13-PGE1
0.11 ± 0.05*
0.07 ± 0.03*
0.78 ± 0.14‡
13-PGE2
14.34 ± 1.62*
7.71 ± 0.94‡
13-HODE
14.05 ± 2.63
23.18 ± 7.63
9.25 ± 1.67
5-HETE
1.04 ± 0.16*
2.65 ± 1.35*
0.12 ± 0.01‡
12-HETE
6.47 ± 1.88*
47.66 ± 12.84‡
2.57 ± 1.40*
15-HETE
1.06 ± 0.10
3.04 ± 1.50
0.11 ± 0.02
AEA
0.59 ± 0.04*
0.52 ± 0.07*
0.04 ± 0.00‡
2-AG
34.88 ± 6.74*
56.17 ± 12.53*
2.32 ± 0.57‡
Mean values ± SEM of TNBS-treated Wt, Het, and Null Fads1 mice (/genotype). Values not sharing a superscript symbol differ (). PG, prostaglandin; TX, thromboxane; 13-PGE1, 13,14-dihydro 15-keto-PGE1; 13-PGE2, 13,14-dihydro 15-keto-PGE2; HODE, hydroxyl-octadecadienoic acid; HETE, hydroxyl-eicosatetraenoic acid; AEA, arachidonoyl ethanolamine; and AG, arachidonoyl glycerol.