Research Article

Antagonizing Arachidonic Acid-Derived Eicosanoids Reduces Inflammatory Th17 and Th1 Cell-Mediated Inflammation and Colitis Severity

Table 2

Colonic mucosal eicosanoid and cannabinoid profiles in Fads1 TNBS-treated mice1.

Eicosanoid
(ng/mg protein)
WtHetNull

PGE11.91 ± 1.09*1.87 ± 0.50*16.36 ± 6.88
PGE215.70 ± 4.92*14.19 ± 1.66*1.92 ± 0.77
PGE30.00 ± 0.000.00 ± 0.000.00 ± 0.00
PGD243.12 ± 4.51*39.14 ± 2.51*1.38 ± 0.06
5.94 ± 0.98*4.15 ± 0.57*0.11 ± 0.03
6-keto- 3.78 ± 0.98*3.00 ± 0.74*0.41 ± 0.11
TXB21.08 ± 0.23*1.26 ± 0.21*0.17 ± 0.02
13-PGE10.11 ± 0.05*0.07 ± 0.03*0.78 ± 0.14
13-PGE214.34 ± 1.62*7.71 ± 0.94
13-HODE14.05 ± 2.6323.18 ± 7.639.25 ± 1.67
5-HETE1.04 ± 0.16*2.65 ± 1.35*0.12 ± 0.01
12-HETE6.47 ± 1.88*47.66 ± 12.842.57 ± 1.40*
15-HETE1.06 ± 0.103.04 ± 1.500.11 ± 0.02
AEA0.59 ± 0.04*0.52 ± 0.07*0.04 ± 0.00
2-AG34.88 ± 6.74*56.17 ± 12.53*2.32 ± 0.57

Mean values ± SEM of TNBS-treated Wt, Het, and Null Fads1 mice ( /genotype). Values not sharing a superscript symbol differ ( ). PG, prostaglandin; TX, thromboxane; 13-PGE1, 13,14-dihydro 15-keto-PGE1; 13-PGE2, 13,14-dihydro 15-keto-PGE2; HODE, hydroxyl-octadecadienoic acid; HETE, hydroxyl-eicosatetraenoic acid; AEA, arachidonoyl ethanolamine; and AG, arachidonoyl glycerol.