Inhibition of Age-Related Cytokines Production by ATGL: A Mechanism Linked to the Anti-Inflammatory Effect of Resveratrol

Figure 1

Failure of ATGL/PPARα upregulation is associated with increased IL-6 in vAT of 24-month-old mice. (a) Upper panel: western blotting analysis of ATGL in total protein extracts from mice vAT at different age (𝑛=3 mice/group). Lower panel: the content of ATGL protein was quantified by densitometric analysis. Data are expressed as ATGL/β-actin. Values are given as means ± S.D. ^(a)𝑃<0.05 versus 1-month-old mice; ^(b)𝑃<0.05 versus 7- and 14-month-old mice. (b) RT-qPCR analysis of relative ATGL, PPARα, and IL-6 mRNA levels in vAT of 3-month- and 24-month-old mice (𝑛=3 mice/group). Values are given as means ± S.D. ^(a)𝑃<0.05 versus 3-month-old mice. (c) Upper panel: western blotting analysis of CD-14 in total protein extracts from vAT of 3-month- and 24-month-old mice (𝑛=3 mice/group). Lower panel: the content of CD-14 protein was quantified by densitometric analysis. Data are expressed as CD-14/Ponceau. β-Actin and Ponceau-stained membranes were used as loading controls.