Inhibition of Age-Related Cytokines Production by ATGL: A Mechanism Linked to the Anti-Inflammatory Effect of Resveratrol

<table>RSV does not revert inflammatory markers upregulation in ATGL lacking cells. (a) Differentiated 3T3-L1 adipocytes (8-days-old) were transfected with siRNA against ATGL [ATGL<svg height="15.0875" id="M16" style="vertical-align:-2.3205pt" version="1.1" viewbox="0 0 21.862499 15.0875" width="21.862499" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>] or with a scramble siRNA (Scr) and treated with 100 <i>μ</i>M RSV for 24 h. RT-qPCR analysis of relative ATGL, PPAR<i>α</i>, TNF<i>α</i>, and IL-6 mRNA levels. Values are given as means ± S.D. <svg height="16.9375" id="M17" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 72.787498 16.9375" width="72.787498" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> versus Scr cells; <svg height="16.9375" id="M18" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 74.550003 16.9375" width="74.550003" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> versus RSV untreated cells. (b) Western blotting analysis of I<i>κ</i>B and phosphoactive form of NF<i>κ</i>B (pNF<i>κ</i>B) in total protein extracts of adipocytes. <i>β</i>-Actin was used as loading control. Immunoblots are representative of at least 3 independent experiments.</table>

Mediators of Inflammation

fig3

Figure 3

Figure 3: Inhibition of Age-Related Cytokines Production by ATGL: A Mechanism Linked to the Anti-Inflammatory Effect of Resveratrol 