Glucagon-Like Peptide-1 Secreting Cell Function as well as Production of Inflammatory Reactive Oxygen Species Is Differently Regulated by Glycated Serum and High Levels of Glucose
Figure 8
GS abrogates insulin-induced phosphorylation of IRS-1(Tyr-895) at both low and high glucose concentration. After 5-day treatment in the presence of standard medium (CTR) or high glucose concentration (25 mmol/L) (HG) in presence of different concentrations of glycated serum (400 [GS] and 800 nmol/L [2GS]), GLUTag cells were incubated for 2 hours in serum-free medium and, then, exposed in the presence (dark bars) or absence (white bars) of 100 nmol/L insulin for 5 min. (a) Representative western blot analysis. (b) Quantification of densitometries of western blot bands. Data were expressed as mean ± SE of fold induction relative to β-actin (). and versus absence of insulin; n.s.: nonsignificant.
