Hydrogen-Rich Saline Inhibits NLRP3 Inflammasome Activation and Attenuates Experimental Acute Pancreatitis in Mice
Figure 2
Evaluation of pancreatic NF-κB activation in mice. The HRS at various doses or control saline (CS) was injected intraperitoneally to AP mice 1 h after the final injection of cerulein, respectively. The pancreatic tissue samples were collected from sacrificed animals in each group () 24 h after the initiation of AP and then homogenized on ice. After the measurement of protein concentration, NF-κB activation was evaluated by measuring the DNA-binding activity of NF-κB p65 in nuclear extracts using an ELISA kit (a) and by detecting the protein levels of I-κBα and p-I-κBα in pancreatic homogenates (b). L: low dose (2 mL/kg × 3); M: middle dose (4 mL/kg × 3); H: high dose (8 mL/kg × 3).
