Enhanced Apoptosis of Monocytes from Complication-Free Juvenile-Onset Diabetes Mellitus Type 1 May Be Ameliorated by TNF-α Inhibitors
Figure 3
Effect of TNF-R1 modifiers on CD16− and CD16+ patients’ monocytes apoptosis. Blood was taken from 10 DM1 patients. Peripheral blood mononuclear cells were isolated and incubated for 24 h in culture medium either without additives or with one of the following substances: anti-TNF-R1 mAb (10 μg/mL), 4-bromophenacyl bromide (BPB) (16 μM), and sodium salicylate (SS) (10 mM). After incubation the cells were stained for visualization of CD14, CD16, and Annexin V binding molecules on monocytes. The percentage of CD16− and CD16+ apoptotic cells was summarized from 3 experiments and presented as arithmetic mean ± S.D. Statistical significance was calculated with Kruskal-Wallis Anova test. Statistical significance for CD16− monocytes: BPB (); statistical significance for CD16+ monocytes: anti-TNF-R1 mAb (), SS (), and PBP . Anti-TNF-R1: mAb against TNF-R1 receptor; APOP: apoptotic BPB: 4-bromophenacyl bromide; SS: sodium salicylate.
