Enhanced Apoptosis of Monocytes from Complication-Free Juvenile-Onset Diabetes Mellitus Type 1 May Be Ameliorated by TNF-α Inhibitors

Figure 4

Gating strategy used to analyze apoptosis of monocyte subsets. The monocyte subset was identified according to the expression of the CD14 molecule and SSC parameter (a). Based on the expression of the CD14 and CD16 molecules, two monocyte subsets were distinguished: CD14⁺⁺CD16⁻ and CD14⁺CD16⁺ cells (b). Monocytes in CD14⁺⁺CD16⁻ (c) and CD14⁺CD16⁺ (d) gates were gated on TNF-R1⁺ cells. Plots (e) and (f) show apoptotic Annexin V-positive CD14⁺⁺CD16⁻TNF-R1⁺ and CD14⁺CD16⁺TNF-R1⁺ cells, respectively.